Research interest:
Mucosal Immunology and Clinical Pathology.
Research summary:
Dr. Burkhard’s research focuses on the mucosal transmission and immunopathogenesis of lentiviruses such as human immunodeficiency virus (HIV). Current studies are directed towards understanding viral latency and the effect of mucosal alloantigen exposure. Additional areas of interest include immunophenotyping of canine and feline lymphoid malignancies, identification of hematologic and immunologic features of disease in fresh water mussels, and evaluation of immune function in veterinary species.
Selected publications:
Howard KE, Burkhard MJ. Mucosal challenge with cell-associated or cell-free FIV induces rapid and distinctly different patterns of phenotypic change in the mucosal and systemic immune systems. Immunology, in press.
Howard KE, Burkhard MJ. FIV infection induces unique changes in phenotype and cellularity in the medial iliac lymph node and intestinal IEL. AIDS Res Hum Retroviruses, 23:720-8, 2007.
Assogba BD, Leavell SE, Porter K, Burkhard MJ. Mucosal administration of low dose cell-associated feline immunodeficiency virus promotes viral latency. J Infect Dis, 195:1184-1188, 2007.
Howard KE, Fisher IL, Dean GA, Burkhard MJ. Methodology for isolation and phenotypic characterization of feline small intestinal leukocytes. Journal Immunological Methods 302:36-53, 2005.
Burkhard MJ, Hoover EA. IgG from acutely infected cats blocks mucosal feline immunodeficiency virus infection. Vet Immunol Immunopathol, 2005; 106:87-95.
Leavell SE, Wright BM, Scappino LA, Sirriyah J, Chen C, Clements JD, Burkhard MJ. Induction of serum and mucosal FIV-specific immune responses by intranasal immunization with p24Gag. Vaccine, 2005; 23:1471-8.
Sirriyah J, Dean GA, LaVoy A, Burkhard MJ. Assessment of CD4+ and CD8+ IFN-gamma producing cells by ELISpot in naïve and FIV-infected cats. Vet Immuno Immunopath 2004; 102:77-84.
Stevens R, Howard KE, Nordone S, Burkhard MJ, Dean GA. Oral immunization with recombinant Listeria monocytogenes protects against vaginal challenge with feline immunodeficiency virus. J Virol 2004; 78:8210-8218.
Burkhard MJ, Dean GA. Transmission and immunopathogenesis of FIV in cats as a model for HIV. Current HIV Research 2003; 1:15-29.
Burkhard MJ, Valenski L, Leavell S, Dean GA, Tompkins WAF. Evaluation of FIV protein-expressing VEE-replicon vaccine vectors in cats. Vaccine 2002; 21:258-68.
Burkhard MJ, Mathiason CK, Bowdre T, Hoover EA. Feline immunodeficiency virus gag- and env-specific immune responses after vaginal versus intravenous infection. AIDS Res Human Retrovir 2001;17:1767-78.
O'Neil LL, Burkhard MJ, Obert LA, Hoover EA. Regression of feline immunodeficiency virus infection. AIDS Res Human Retrovir 1997; 13:713-718.
Burkhard MJ, Obert LA, O'Neil LL, Diehl LJ, Hoover EA. Mucosal transmission of cell-associated and cell-free feline immunodeficiency virus. AIDS Res Human Retrovir 1997; 13:347-355.
O'Neil LL, Burkhard MJ, Hoover EA. Frequent perinatal transmission of feline immunodeficiency virus by chronically infected cats. J Virol 1996; 70:2894-2901.
O'Neil LL, Burkhard MJ, Diehl LJ, Hoover EA. Vertical transmission of feline immunodeficiency virus. AIDS Res Human Retrovir 1995; 11:171-182.
