Research Interest:
Genomic alterations in cancer
Research Summary:
The goal of the Nucleic Acid Shared Resource is to facilitate cancer research by providing a high quality, fast, cost effective and reliable support for the OSU Comprehensive Cancer Center. Specifically, the Nucleic Acid Shared Resource provides instrumentation and expertise for DNA sequencing, genotyping, real-time PCR, RNA/DNA extraction, imaging, and DNA synthesis support. The laboratory also offers expertise for consulting and assisting in experimental designs to investigators. Additionally, the facility supports the development of novel methodologies and applications relevant to cancer research and it functions as a training and education center.
Selected Publications:
Mori, M., Mimori, K., Shiraishi, T., Alder, H., Inoue, H., Tanaka, Y., Sugimachi, K., Huebner, K. and Croce, C.M.: Altered expression of Fhit in Carcinoma and precarcinomatous lesions of the esophagus. Cancer Res., 60, 1177-1182, 2000.
Ishii, H., Dumon, K., Vecchione, A., Trapasso, F., Mimori, K., Alder, H., Mori, M., Sozzi, G., Baffa, R., Huebner, K. and Croce, C.M.: Effect of adenoviral transduction of FHIT into esophageal cancer cells. Cancer Res., 61, 1578-1584, 2001.
Kalman, B., Chatterjee, D., Lu, F., Alder, H.: Mitochondrial DNA Variants In Multiple Sclerosis. Chapter 20. In: Mitochondrial Ubiquinone (Coenzyme Q10): Biochemical, Functional, Medical, and Therapeutic Aspects in Human Health and Diseases. Volume 2. Eds.: Manuchair Ebadi, Joe Marwah, and Raj K. Chopra. Prominent Press, 2001.
Mabuchi, H., Fujii, H., Calin, G., Alder, H., Negrini, M., Bullrich, F. and Croce, C.M.: Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia. Cancer Res., 61, 2870-2877, 2001.
Shiraishi, T., Druck, T., Mimori, K., Flomenberg, J., Berk, L., Alder, H., Huebner, K. and Croce, C.M.: Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/Fhit and Fra14A2/Fhit. Proc. Natl. Acad. Sci., USA, 98: 5722-5727, 2001.
Vecchione, A., Ishii, H., Shiao, Y.-H., Trapasso, F., Rugge, M., Alder, H., Croce, C.M. and Baffa, R.: Fez1/Lzts1 alterations in gastric carcinoma. Clin. Cancer Res., 7: 1546-1552, 2001.
Ishii, H., Vecchione, A., Baldassarre, G., Murakumo, Y., Alder, H., Baffa, R. and Croce, C.M.: Fez1/Lzts1 gene at 8p22 suppresses cancer cell growth and regulates mitosis. Proc. Natl. Acad. Sci., USA, 98: 10374-10379, 2001.
Bullrich, F., Fujii, H., Calin, G., Mabuchi, H., Negrini, M., Pekarsky, Y., Rassenti, L., Alder, H., Reed, J.C., Keating, M.J., Kipps, T.J. and Croce, C.M.: Characterization of the 13q14 tumor suppressor locus in CLL. Identification of ALT1, an alternative splice variant of the LEU2 gene. Cancer Res., 61: 6640-6648, 2001.
Mimori, K., Inoue, H., Alder, H., Ueo, H., Tanaka, Y.-I., and Mori, M.: Mutation analysis of hBUB1, human mitotic checkpoint gene in multiple carcinomas. Oncology Reports 8: 39-42, 2001.
Vecchione, A., Ishii, H.,Baldassarre, G., Bassi, P.F.,Trapasso, F., Alder, H., Pagano, F., Gomella, L.G., Croce, C.M. and Baffa, R.: FEZ1/LSTS1 is down-regulated in high-grade bladder cancer, and its restoration suppresses tumorigenicity in transitional cell carcinoma cells. Amer. J. Pathol., 160:1345-1352, 2002.
Kuroki, T., Trapasso, F., Shiraishi, T., Alder, H., Mimori, K., Mori,M. and Croce, C.M.: Genetic alterations of the tumor suppressor gene WWOX in esophagal squamous cell carcinoma. Cancer Res. 62: 2258-2260, 2002.
Calin, G.A., Dumitru, C.D., Shimizu, M., Bichi,R., Zupo, S., Noch, E., Alder, H., Rattan, S., Keating, M., Rai, K., Rassenti, L., Kipps, T., Negrini, M., Bullrich, F., and Croce, C.M.: Frequent deletions and down-regulation of micro RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc. Natl. Acad. Sci., USA, 99: 15524-15529, 2002.
Kuroki, T., Trapasso, F., Yendamuri, S., Matsuyama, A., Alder, H., Mori, M., and Croce, C.M.: Promoter hypermethylation of RASSF1A in esophagal squamous cell carcinoma. Clin. Cancer Res., 9: 1441-1445, 2003.
Cesari, R., Martin, E.S., Calin, G.A., Pentimalli, F., Bichi, R., McAdams, H., Trapasso, F., Drusco, A., Shimizu, M., Masciullo, V., d’Andrilli, G., Scambia, G., Picchio, M.C., Alder, H., Godwin, A.K., and Croce, C.M.: Parkin, a gene implicated in autosomal recessive juvenile Parkinsonism, is a candidate tumor suppressor gene on chromosome 6q25-q27. Proc. Natl. Acad. Sci., USA, 100: 5956-5961, 2003.
Thakur, S., Nakamura, T., Calin, G., Russo, A., Tamburrino, J.F., Shimizu, M., Baldassarre, G., Battista, S., Fusco, A., Wassell, R.P., DuBois, G., Alder, H., and Croce, C.M.: Regulation of BRCA1 transcription by specific single-stranded DNA binding factors. Mol. Cell. Biol., 23: 3774-3787, 2003.
Kuroki, T., Trapasso, F., Yendamuri, S., Matsuyama, A., Alder, H., Mori, M., and Croce, C.M.: Allele loss and promoter hypermethylation of VHL, RAR-b, RASSF1A, and FHIT tumor suppressor genes on chromosome 3p in esophageal squamous cell carcinoma. Cancer Res., 63: 3724-3728, 2003.
Kuroki, T., Trapasso, F., Yendamuri, S., Matsuyama, A., Alder, H., Williams, N.N., Kaiser, L.R., and Croce, C.M.: Allelic loss on chromosome 3p21.3 and promoter hypermethylation of SEMA3B in non-small cell lung cancer. Cancer Res., 63: 3352-3355, 2003.
Matsuyama, A., Shiraish, T., Trapasso, F., Alder, H., Mori, M., Huebner, K., and Croce, C.M.: Genome stability and fragility: fragile site orthologs FHIT/FRA3B and Fhit/Fra14A1 are evolutionarily conserved but mitotically unstable. Proc. Natl. Acad. Sci., USA, 10: 14988-14993, 2003.
Kuroki, T., Yendamuri, S., Trapasso, F., Matsuyama, A., Aqueilan, R.I., Alder, H., Rattan, S., Cesari, R., Nolli, M.L. Williams, N.N., Mori, M., Kanematsu, T., and Croce, C.M.: The tumor suppressor gene WWOX at FRA16D is involved in pancreatic carcinogenesis. Clin. Cancer Res., 10: 2459-2465, 2004.
Liu, C.-G., Calin,G.A., Meloon, B., Gamliel,N., Sevignani, C., Ferracin, M., Dumitri, C.D., Shimizu, M., Zupo, S., Dono, M., Alder, H., Bullrich, F., Negrini, M., and Croce, C.M.: An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Proc. Natl. Acad. Sci., USA, 101: 9740-9744, 2004.
Calin, G.A., Liu, C.G., Sevignani, C, Ferracin, M., Felli, N., Dumitru, C.D., Shimizu, M., Cimmino, A., Zupo, S., Dono, M., Del’Aquila, M.L., Alder, H., Rassenti, L., Kipps, T.J., Bullrich, F., Negrini, M., Croce, C.M.: MicroRNA profiling reveals distinct signatures in B-cell chronic lymphocytic leukemias. Proc. Natl. Acad. Sci. USA,101:11755-60, 2004.
Trapasso, F., Sarti, M., Cesari, R., Yendamuri, S., Dumon, K. R., Aqeilan, R. I., Pentimalli, F., Infante, L., Alder, H., Abe, N., Watanabe, T., Viglietto, G, Croce, C. M., and Fusco, A.: Therapy of human pancreatic carcinoma based on suppression of HMGA1 protein synthesis in preclinical models. Cancer Gene Ther 11(9): 633-641, 2004.
Calin, G.A., Trapasso, F., Shimizu, M., Dumitru, C.D., Yendamuri, S., Godwin, A.K., Ferracin, M., Bernardi, G., Chatterjee, D., Baldassarre, G., Rattan, S., Alder, H., Mabuchi, H., Shiraishi, T., Hansen, L.L., Overgaard, M., Herlea, V., Mauro, F.R., Dighiero, G., Movsas, B., Rassenti, L., Kipps, T., Baffa, R., Fusco, A., Mori, M., Russo, G., Liu, C.-G., Neuberg, D., Bullrich, F., Negrini, M., and Croce, C.M.: Familial Cancer associated with a polymorphism in ARLTS1. N Engl J Med 352, 16: 1667-1676, 2005.
Cimmino, A., Calin, G.A., Fabbri, M., Iorio, M.L., Ferracin, M., Shimizu, M., Wojcik, S.E., Aqeilan, R., Zupo, S., Dono, M., Rassenti, L., Alder, H., Volinia, S., Liu, C.-G., Kipps, T.J., Negrini, M., and Croce, C.M.: MiR-15 and miR-16 induce apoptosis by targeting BCL2. Proc. Natl. Acad. Sci. USA,102:13944-49, 2005.
Calin, G.A., Ferracin, M., Cimmino, A., DiLeva, G., Shimizu, M., Wojcik, S.E., Iorio, M.V., Visone, R., Sever, N.I., Fabbri, M., Iuliano, R., Palumbo, T., Pichiorri, F., Roldo, C., Garzon, R., Sevignani, C., Rassenti, L., Alder, H., Volinia, S., Liu, C.-G., Kipps, T., Negrini, M., and Croce, C.M.: A microRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med 353, 1793-1801, 2005.
