Research Assistant Professor
Department of Molecular Virology, Immunology, and Medical Genetics
385P Wiseman Hall
400 West 12th Ave.
Columbus, OH 43210
Ph. (614) 247-8397
B.S. Biology 1993 Massachusetts Institute of Technology
Ph.D. Biology 2000 University of California, San Diego
Integration of the retroviral genome into a host chromosome is an essential part of the HIV life cycle. However, understanding of integration is still incomplete. Our studies of host DNA repair factors have identified both positive and negative regulators of retroviral infection. Proteins of the nucleotide excision repair pathway, XPB and XPD, are able to defend the host genome from retroviral integration by degrading the viral cDNA by an evolutionarily ancient mechanism. In contrast, base excision repair (BER) proteins enhance HIV integration. We are continuing to explore the role of BER proteins during integration. Finally, we are evaluating the role of chromatin during integration. Integrase favors DNA wrapped around nucleosomes far more than naked DNA. These studies provide fundamental knowledge of integration that may be useful for enhancing anti-retroviral therapy and also for targeting integration of retroviral gene therapy vectors.
For current publications please visit The National Center for Biotechnology Information's PubMed website at http://www.ncbi.nlm.nih.gov/pubmed?term=yoder%2C%20kristine
Yoder, K.E.*, A. Espeseth, X. Wang, Q. Fang, M.T. Russo, R.S. Lloyd, D. Hazuda, R.W. Sobol, and R. Fishel. 2011. The host base excision repair pathway is required for efficient lentivirus integration. PLoS ONE. 6:e17862. *corresponding author
Espeseth, A.S., R. Fishel, D. Hazuda, Q. Huang, M. Xu, K. Yoder, and H. Zhou. 2011. siRNA screening of a targeted library of DNA repair factors in HIV infection reveals a role for base excision repair in HIV integration. PLoS ONE. 6:e17612.
Yoder, K.E.*, W. Roddick, P. Hoellerbauer, and R. Fishel. 2011. XPB mediated retroviral cDNA degradation coincides with entry to the nucleus. Virology. 410:291-298. *corresponding author
Charbonneau, N., R. Amunugama, C. Schmutte, K. Yoder, and R. Fishel. 2009. Evidence that hMLH3 functions primarily in meiosis and in hMSH2-hMSH3 mismatch repair. Cancer Biology and Therapy. 8:1411-1420.
Yoder, K.E.*, and R. Fishel. 2008. Real-time quantitative PCR and Fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles. Journal of Virological Methods. 153:253-256. *corresponding author
Ikura, T., S. Tashiro, A. Kakino, H. Shima, N. Jacob, R. Amunugama, K. Yoder, S. Izumi, I. Kuraoka, K. Tanaka, H. Kimura, M. Ikura, S. Nishikubo, T. Ito, A. Muto, K. Miyagawa, S. Takeda, R. Fishel, K. Igarashi, and K. Kamiya. 2007. DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics. Molecular and Cellular Biology. 27:7028-7040.
Yoder, K., A. Sarasin, K. Kraemer, M. McIlhatton, F. Bushman, and R. Fishel. 2006. The DNA Repair Genes XPB and XPD Defend Cells from Retroviral Infection. Proceedings of the National Academy of Sciences. 103:4604-4609.
Shim, K-S., C. Schmutte, K. Yoder, and R. Fishel. 2006. Defining the mechanism of salt-induced human RAD51 activities. DNA Repair. 5:718-730.
Yoder, K.E.*, and R. Fishel. 2006. PCR based detection is unable to consistently distinguish HIV 1LTR circles. Journal of Virological Methods. 138:201-206. *corresponding author
Picchio, M.C., E.S. Martin, R. Cesari, G.A. Calin, S. Yendamuri, T. Kuroki, F. Pentimalli, M. Sarti, K. Yoder, L.R. Kaiser, R. Fishel, and C.M. Croce. 2004. Alterations of the tumor suppressor gene Parkin in non-small cell lung cancer. Clinical Cancer Research. 10:2720-2724.
Olson, P.D., K. Yoder, L.F. Fajardo, A.M. Marty, S. van de Pas, C. Olivier, and D.A. Relman. 2003. Lethal invasive cestodiasis in immunosuppressed patients. Journal of Infectious Diseases. 187:1962-1966.
Martin, E.S., R. Cesari, F. Pentimalli, K. Yoder, R. Fishel, A.L. Himelstein, S.E. Martin, A.K. Godwin, M. Negrini, and C.M. Croce. 2003. The BCSC-1 locus at chromosome 11q23-q24 is a candidate tumor suppressor gene. Proceedings of the National Academy of Sciences. 100:11517-11522.
Li, L., J.M. Olvera, K.E. Yoder, R.S. Mitchell, S.L. Butler, M. Lieber, S.L. Martin, and F.D. Bushman. 2001. Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection. EMBO Journal. 20:3272-3281.
Olivier, C., S. van de Pas, P.W. Lepp, K. Yoder, and D.A. Relman. 2001. Sequence variability in the first internal transcribed spacer region within and among Cyclospora species is consistent with polyparasitism. International Journal for Parasitology. 31:1475-1487.
Yoder, K.E. and F.D. Bushman. 2000. Repair of gaps in retroviral DNA integration intermediates. Journal of Virology. 74:11191-11200.
Li, L., K.E. Yoder, M.S.T. Hansen, J. Olvera, M.D. Miller, and F.D. Bushman. 2000. Retroviral cDNA integration: Stimulation by HMG I-family proteins. Journal of Virology. 74:10965-10974.
Relman, D.A., D.N. Fredericks, K.E. Yoder, G. Mirowski, T. Berger, and J.E. Koehler. 1999. Absence of Kaposi's sarcoma-associated herpesvirus DNA in bacillary angiomatosis-peliosis lesions. Journal of Infectious Diseases. 180:1386-1389.
Fortunato, E.A., M.H. Sommer, K. Yoder, and D.H. Spector. 1997. Identification of domains within the human cytomegalovirus major immediate-early 86-kilodalton protein and the retinoblastoma protein required for physical and functional interaction with each other. Journal of Virology. 71: 8176-8185.
Relman, D.A., T.M. Schmidt, A. Gajadhar, M. Sogin, J. Cross, K. Yoder, O. Sethabutr, and P. Echeverria. 1996. Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species. Journal of Infectious Diseases. 173: 440-445.