Paul M.L. Janssen, Ph.D.

Assistant Professor 200/206 Hamilton Hall 1645 Neil Avenue Columbus, Ohio  43210-1218 janssen.10@osu.edu

Education:
Ph.D., combined program between Wake Forest University, NC, University of Illinois at Chicago, and Utrecht University, the Netherlands.

Research Interests:
The main research interests in my lab are the mechanisms that govern and/or impact on cardiac relaxation in health and disease. Other research areas include the functional effects of hydroxyl radical damage on the myocardium, load dependent hypertrophy, cardiopharmacology, and skeletal muscular dystrophy.

Techniques Available:
We use a wide variety of techniques to study myocardial relaxation, from the single cell level to the intact heart. Our main preparation is the multicellular trabeculae. These ultra-thin muscles (about 100 µm in diameter), possess all major cardiac cell types, and it's contractile behavior very closely mimics that of the whole heart.

In cardiac tissue, we can measure intracellular calcium transients, as well as pH, under physiological temperature and frequency, in a variety of species, and we can keep these intact beating trabeculae alive and beating for several days. In addition, using confocal and 2-phoon imaging, we are able to study myocyte dimensions under intact conditions. We have several models of disease, including pulmonary artery banding, and aortic banding. In collaborative studies, we assess skeletal muscle function under a variety of conditions.

Collaborators: The Janssen lab actively collaborates with other departmental faculty, other faculty at OSU, and faculty at other institutions:
Dr. Mark Ziolo
Dr. Muthu Periasamy
Dr. Jonathan Davis
Dr. George Billman
Dr. Sandor Gyorke
Dr. Jill Rafael-Fortney (Mol. Cell. Biochem)
Dr. Bill Abraham (Cardiology)
Dr. David Feldman (Cardiology)
Dr. Robert Hamlin (Veterinary Medicine) 
Dr. Paul Martin (Nationwide Children’s Hospital)
Dr. Jerry Mendell (Nationwide Children’s Hospital)
Dr. Federica Montanaro (Nationwide Children’s Hospital)  
Dr. Brian Kaspar (Nationwide Children’s Hospital)  

External Funding:
• NIH (NHLBI) R01 HL073816, 04/01/2004-03/31/2009, Janssen (PI)
• NIH (NHLBI) KO2 HL083957, 04/01/2006-03/31/2011, Janssen (PI)
• AHA National Center, Established Investigator Award 0740040N, 01/01/2007-12/31/2011, Janssen (PI)
• NIH NINDS U54, 09/01/2007-08/31/2011, Mendell (PI), Janssen (Co-PI)
• NIH NCI UO1, 01/12/2007-11/30/2010, Guttride (PI), Janssen (Co-PI)
• American Heart Association Ohio Valley Affiliate, Pre-doctoral award, 01/07/2006-06/30/2008, Varian (PI), Janssen (Mentor)
• American Heart Association Great Rivers Affiliate, Pre-doctoral award, 07/01/2007-06/30/2009, Hiranandani (PI), Janssen (Mentor)
• Emergency Medicine Foundation, Career development award, 07/1/2007-06/30/2008, Torres (Student-PI), Janssen (Mentor)

Publications:
• Varian KD, Janssen PML. Frequency dependent acceleration of relaxation involves decreased myofilament calcium sensitivity. Am. J. Physiol. Heart. Circ. Physiol. 2007;292:H2212-2219.
• Acharyya S, Villalta SA, Bakkar N, Bupha-Intr T, Janssen PML, Carathers M, Li Z, Ghosh S, Sahenk Z, Weinstein M, Gardner KL, Rafael-Fortney JA, Karin M, Tidball JG, Baldwin AS, Guttridge DC. IKK/NF-kB Signaling interplay in macrophages and myofibers promotos muscle generation in Duchenne muscular dystrophy. J. Clin. Invest. 2007;117:889-901.
• Hiranandani N, Raman S, Kalyanasundaram A, Periasamy M, Janssen PML. Frequency-dependent contractile strength in mice over- and under-expressing the sarcoplasmic reticulum calcium ATPase. Am. J. of Physiol. Reg. Integr. Comp. Physiol. 2007;293:R30-R36.
• Keweloh B, Janssen PML, Siegel U, Datz N, Zeitz O, Hermann HP. Influence of pyruvate on economy of contraction in isolated rabbit myocardium. Eur. J. Heart Fail. 2007;9:754-761.
• Carnes CC, Janssen PML, Ruehr ML, Nakayama H, Nakayama T, Haase H, Bauer JA, Chung MK, Fearon IM, Gillinov MA, Hamlin RL, Von Wagoner DR. Atrial Glutathione Content, Calcium Currents and contractility. J. Biol. Chem. 2007;282:28063-28073.
• Janssen PML, Periasamy P. Determinants of Frequency Dependent Contraction and Relaxation of Mammalian Myocardium. J Mol Cell Cardiol. 2007;43:523-531 (review).
• Rodino-Klapac LR, Janssen PML, Montgomery CL, Coley BD, Chicoine LG, Clark KR, Mendell JR. A translational approach for limb vascular delivery of the micro-dystrophin gene without high volume or high pressure treatment. J. Translational Medicine 2007;5:45.
• Guterl KA, Haggart CR, Janssen PML, Holmes JW. Isometric contraction induces rapid myocyte remodeling in cultured rat right ventricular papillary muscles. Am J. Physiol. Heart Circ. Physiol. 2007;293:H3707-H3712.
• Bupha-Intr T, Holmes JW, Janssen PML. Induction of hypertrophy in vitro by mechanical loading in adult rabbit myocardium. Am J. Physiol. Heart Circ. Physiol. 2007;293:H3759-H3767.
• Monasky MM, Varian KD, Janssen PML. Gender comparison of contractile performance and beta-adrenergic response in isolated rat cardiac trabeculae. J. Comp. Physiol. Biochem.,(in press), 2007.
• Monasky MM, Varian KD, Davis JP, Janssen PML. Dissociation of Force Decline from Calcium Decline by Preload in Isolated Rabbit Myocardium. Eur J Physiol., (in press), 2007.
• Periasamy M, Janssen PML. Molecular basis of diastolic dysfunction. Heart Failure Clinics. 2008 (in press) (review).