Dale D. Vandre, Ph.D.
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Associate Professor
Associate Director, College of Medicine Preclinical Med 1/2 Integrative Program
002 Hamilton Hall
1645 Neil Avenue
Columbus OH 43210-1218
vandre.1@osu.edu |
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Education:
Ph.D., Biochemistry, University of Iowa, Iowa City, OH
In addition to my research laboratory (see below), I am also involved in educational administration in the College of Medicine. I am currently the Associate Director of the Preclincal Med 1/2 Integrated Program. This is the largest educational pathway chosen by incoming medical students, and is based upon lectures, small group discussion, and team based learning activities. As part of my educational responsibilities in the medical curriculum, I am the Director of the Cell Block, which provides the 1st year student with a foundation in biochemistry, cell biology, histology, pathology, pharmacology, and medical genetics focused around neoplastic disease. My specific lectures include tissue histology, angiogenesis, cell cycle regulation, oncogenes, and cell growth regulation. As of July 1st 2008, I will become the Director of the Preclincal Med 1/2 Integrated Program.
OTHER RESPONSIBILITIES
Vice President of the College of Medicine Faculty Council 2005-2007
President of the College of Medicine Faculty Council 2007-2009
College of Medicine Committees
Academic Program Committee
Student review Subcommittee
Executive Curriculum Committee
University Committees
University Research Committee
Mass Spec & Proteomics User Advisory Committee
RESEARCH INTERESTS
My laboratory is involved in utilizing proteomics techniques and approaches to identify novel membrane proteins that are associated with specific tissues, and to determine differences in expression associated with development or disease processes. In particular we have focused our most recent efforts on developing methods for the isolation of highly purified plasma membrane samples that are suitable for proteome analysis. In collaboration with the Robinson lab (also in this Department), we have been successful in preparing and analyzing the proteome of the apical plasma membrane of the human placental syncytiotrophoblast. This membrane is of particular importance, as it forms the interface between the fetal and maternal blood system. As such, proper function of this plasma membrane is required for the transport of nutrients to the developing fetus.
As part of our analysis, we have identified members of the ferlin family of proteins as being highly expressed in the apical syncytiotrophoblast membrane. This is of interest, since ferlin proteins have been shown to be involved in membrane repair functions in skeletal muscle. We are in the process of examining the functional role of these ferlin proteins in placental normal and diseased placentas.
In addition to the labs interest in proteomics and membrane repair, other projects in the lab are related to cell cycle regulation and cytoskeletal organization. We have recently been interested in processes that occur during exit from mitosis and entry into interphase. Shortly after nunclear envelope reformation, the nucleolus is also reassembled. We are examining this process, which is termed nucleologenesis, and the role of nuclear transport in allowing this process to be completed.
Two dimensional polyacrylamide gel separation of proteins isolated from HL-60 humanleukemia cell fractions. Gel provides an example comparison of proteins present in cytoplasmic (red) and isolated plasma membrane (green) fractions. Yellow indicates proteins present in both fractions. The results illustrate the differential localization of proteins within subcellular compartments.
RECENT PUBLICATIONS
• Robinson JM, Ackerman WE 4th, Kniss DA, Takizawa T, Vandré DD. Proteomics of the human placenta: promises and realities. Placenta 2008 Feb; 29(2):135-43.
• Ackerman WE 4th, Summerfield TL, Vandre DD, Robinson JM, Kniss DA. Nuclear factor-kappa B regulates inducible prostaglandin E synthase expression in human amnion mesenchymal cells. Biol Reprod 2008 Jan; 78(1):68-76.
• Vandré DD, Ackerman IV WE, Kniss DA, Tewari A, Mori M, Takazawa T, Robinson JM. Dysferlin is Expressed in Human Placenta but Does Not Associate With Caveolin. Biol Reprod 2007 Sep; 77(3):533-42.
• Kannanayakal TJ, Tao H, Vandre DD, Kuret J. Casein kinase-1 isoforms differentially associate with neurofibrillary and granulovacuolar degeneration lesions. Acta Neuropathol (Berl) 2006 May; 111(5):413-21.
• Marsee DK, Venkateswaran A, Tao H, Vadysirisack D, Zhang Z, Vandre DD, Jhiang SM. Inhibition of Heat Shock Protein 90, a Novel RET/PTC1-associated Protein Increases Radioiodide Accumulation in Thyroid Cells. J Bio Chem, 279:43990-7, 2004.
• Robinson JM, Takizawa T, Vandré DD. Gold-Cluster Immunoprobes: Light and Electron Microscopy. In: GW Hacker, J Gu (eds.), Gold and Silver Staining: Techniques in Molecular Morphology, CRC Press, Boca Raton, FL, 177-187, 2002.
• Thomas JP, Moore T, Kraut EH, Balcerzak SP, Galloway S, Vandré DD. A Phase II study of CI-980 in Previously Untreated Extensive Small-Cell Lung Cancer: An Ohio State University Phase II Consortium Study. Cancer Invest 20:192-8, 2002.
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