Mark T. Ziolo, Ph.D.

Assistant Professor

019 Hamilton Hall (office)
034 Hamilton Hall (lab)
1645 Neil Avenue
Columbus OH 43210-1218

614-688-7905 (office)
614-688-7900 (lab)
614-688-7999 (fax)
ziolo.1@osu.edu

Education:
Ph.D., Physiology and Biophysics, University of Illinois, Chicago, IL

Research Interests:
My research area is excitation-contraction coupling in the heart using state-of-the-art electrophysiological and optical methods. The focus of my research is how nitric oxide and/or cyclic GMP alter cardiac myocite function. Other areas include b-adrenergic signaling and human heart failure.

Techniques Available:
We use a variety of state-of-the-art techniques in the lab to study cardiac myocyte function- epifluorescence (to measure Ca²⁺ and NO), video edge detection (cell shortening), patch clamp (whole cell voltage clamp for I_Ca, I_Kr, I_Ks, I_K1, NCX and current clamp for action potentials), and confocal microscopy (line scan mode- Ca²⁺ sparks). I have 2 experimental setups in which we use patch clamp, epifluorescence and/or video edge detection and there is a confocal microscope in DHLRI.

Laboratory Environment:
The general area of research for my lab is investigating Excitation-Contraction Coupling (EC Coupling) in the heart. More specifically, I examine the effects of nitric oxide (NO) on ECC. NO is a free radical that is produced in cardiac myocytes via enzymes termed NO synthase (NOS). There are 3 isoforms of NOS, and all 3 are expressed in myocytes. The lab studies the effect of all 3 isoforms on cardiac function. Other areas of interest include the effects of b-adrenergic stimulation and disease states (e.g., heart failure) on cardiac myocyte EC coupling.

Studies performed in the lab are done on isolated cardiac myocytes (the muscle cells of the heart). We isolate the myocytes from a variety of animals- transgenic mice (NOS1^-/-, NOS3^-/-, NOS2^-/-, a cardiac specific, inducible overexpression of NOS2, and others (through collaborations), rabbits (control and adenovirus-mediated overexpression (in culture), failing human hearts (from transplant recipients or LVAD implants) and a canine heart failure model.

Laboratory Members:
Honglan Wang, Ph.D., Postdoctoral Researcher
Christopher Traynham, Graduate Student
Dr. Ziolo
Mark Kohr, Graduate Student

Collaborators:
Paul M.L. Janssen, Ph.D., The Ohio State University
Muthu Periasamy, Ph.D., The Ohio State University
Sandor Gyorke, Ph.D., The Ohio State University
David Feldman, M.D., Ph.D., The Ohio State University
Cynthia Carnes, Ph.D., The Ohio State University
Jeff Molkentin, Ph.D., Cincinnati Children's Hospital Medical Center
Tom Shannon, D.V.M., Ph.D., Rush University
Litsa Kranias, Ph.D., University of Cincinnati
Jay Zweier, M.D., The Ohio State University

Selected Publications:
• Bers DM, Ziolo MT. When Is cAMP Not cAMP?: Effects of Compartmentalization. Circ Res 89(5):373-5, 2001.
• Ziolo MT, Katoh H, Bers DM. Expression of inducible NOS depresses beta-adrenergic-stimulated calcium release from the sarcoplasmic reticulum in intact ventricular myocytes. Circulation 104(24):2961-2966, 2001.
• Ziolo MT, Maier LS, Piacentino III, V, Bossuyt J, Houser SR, Bers DM. Myocyte NOS2 contributes to blunted beta;-adrenergic response in failing human hearts by decreasing Ca2+ transients. Circulation 109:1886-1891, 2004.
• Ziolo MT, Martin JL, Bers DM, Pogwizd SM. Adenoviral gene transfer of mutant phospholamban rescues contractile dysfunction in failing myocytes with relatively preserved SERCA function. Circ Res 96:815-817, 2005.
• Kohr MJ, Wang H, Wheeler DG, Velayutham M, Zweier JL, Ziolo MT. Targeting of phospholamban by peroxynitrite decreases beta-adrenergic stimulation in cardiomyocytes. Cardiovasc Res 77(2):353-361, 2008.
• Feldman DS, Elton TS, Sun B, Martin MM, Ziolo MT. Mechanisms of Disease: Detrimental Adrenergic Signaling in Acute Decompensated Heart Failure. Nat Clin Pract Cardiovasc Med 2008. In Press.
• Wang H, Kohr MJ, Wheeler DG, Ziolo MT. Endothelial Nitric Oxide Synthase Decreases beta-adrenergic Responsiveness via Inhibition of the L-type Ca2+ Current. Am J Physiol-Heart 2008. In Press.
• Ziolo MT. The Fork in the Nitric Oxide Road: Cyclic GMP or Nitrosylation. Nitric Oxide 2008. In Press.

Ongoing Projects:
Effects of nitric oxide and/or cyclic GMP on Excitation-Contraction Coupling in the heart. Other areas include b-adrenergic signaling and human heart failure.