Department of Cancer Biology and Genetics
Director, Genetically Engineered Mouse Modeling Shared Resource
Comprehensive Cancer Center
The Ohio State University Comprehensive Cancer Center
Biomedical Research Tower, Room 988
460 W. 12th Avenue
Columbus, OH 43210
(614) 688 8038 office
(614) 688 5933 fax
Education and training
1991 Doctoral Degree in Medicine and Surgery (honors), Federico II University,Naples, Italy
1992 Residency (Medical Sciences) University of Naples, Italy
1997 Specialization in Oncology (honors), University of Padua, Italy
2000 Post-doctoral Fellowship in Dr. Lino Tessarollo’s Laboratory at the Center for Cancer Research/National Cancer Institute in Frederick, MD, USA
Positions and Appointments
2010-Present: Director Genetically Engineered Mouse Modeling Shared Resource (GEMMSR) of the Ohio State Comprehensive Cancer Center (OSUCCC)
Assistant Professor, Molecular Virology Immunology and Medical Genetics in the College of Medicine of the Ohio State University
2001-2010: Head, Gene Targeting Facility, Mouse Cancer Genetics Program, Center for Cancer Research/National Cancer Institute in Frederick, MD, USA
Genetically Engineered Mouse Modeling Shared Resource
As Director of the GEMMSR, I collaborate with Ohio State University Investigators for designing, generation, and development of Mouse Models for human disease.
For more information about the GEMMSR, please visit: GEMMSR webpage
The elucidation of the physiological and pathological role of Neurotrophins (NTs) and their receptors has been my research interest since 1997, when I joined as a postdoctoral fellow the laboratory of Dr. Lino Tessarollo at the CCR/National Cancer Institute (NCI) in Frederick, MD.NTs got their name from early studies showing that they are essential for the nervous system development and function. However, over the years it has been shown that NTs have pleiotropic biological effects and many cell types other than neurons and glia express their receptors. It is now clear that tumor cells use NTs and their high affinity receptors named Tropomyosin Related Kinase Receptor (Trks) for their proliferative and survival advantage. Therefore, similarly to other tyrosine kinase receptors, in many cancer types Trks are considered oncogenes. My lab is focused on the study of Trk and related signaling molecules that are relevant to cancerogenesis. In particular, my lab is studying a new scaffold protein named RanBPM, that mediates Neurotrophin effects and it may be important in specific types of tumor development.
Techniques and Models used in the Coppola Lab.
The methods we use in my lab include those in the areas of genetics, cell biology, biochemistry, molecular biology, pathology, and physiology. Mouse modeling has a central role in my lab. However, we complement the studies using also cells (primary and cell lines), and clinical samples. Examples of specific techniques:
- Genetically engineering of mouse modeling (mouse ES cell culturing and targeting, BAC reombineering, Southern analyses)
- Molecular biology: Cloning, RNA preparation and analyses (real-time PCR, northern), RNAi, Western blotting, immuno-precipitation.
- Cell biology: analyses of specific cell populations, in situ hybridization, immnuocytochemistry, apoptosis assays, cell cycle analyses, immunofluorescent assay, confocal microscopy.
- Microarray, proteomics, and bioinformatics.
For current publications please visit The National Center for Biotechnology Information's PubMed website at Coppola PubMed
Selected publications (out of 41, as of July 2012)
Padmakumar VC, Speer K, Pal-Ghosh S Masiuk K, Ryscavage A, Dengler S, Hwang S, Edwards J, Coppola V, Tessarollo L, Stepp MA, and Yuspa SH. Spontaneous skin erosions and reduced skin and corneal wound healing characterize CLIC4 null mice. Am J Pathol. 2012 Jul;181(1):74-84. Epub 2012 May 18. PMID:22613027
Diril MK, Ratnacaram CK, Padmakumar VC, Du T, Wasser M, Coppola V, Tessarollo L, Kaldis P. Cyclin-dependent kinase 1 (cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration. Proc Natl Acad Sci USA. 2012;109(10): 3826-31. PMCID:PMC3309725.
Wirth EK, Conrad M, Winterer J, Wozny C, Carlson BA, Roth S, Schmitz D, Bornkamm GW, Coppola V, Tessarollo L, Schomburg L Kohrle J, Hatfield DL, Schweizer U. Neuronal selenoprotein expression is required for interneuron development and prevents seizures and neurodegeneration. FASEB J. 2010; 24(3): 844-52. PMCID:PMC2830140.
Satyanarayana A, Gudmundsson KO, Chen X, Coppola V, Tessarollo L, Keller JR, Hou SX. rapGEF2 is essential for embryonic hematopoiesis but dispensable for adult hematopoiesis. Blood 2010 July 1. PMCID: PMC2974602.
Tessarollo L, Palko ME, Akagi K, Coppola V. Gene targeting in mouse embryonic stem cells. Methods Mol Biol. 2009;530:141- 64.
Satyanarayana A, Berthet C, Lopez-Molina J, Coppola V, Tessarollo L, Kaldis P. Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. Development. 2008;135(20):3389-400. PMCID:PMC2668819.
Coppola V, Barrick CA, Bobisse S, Rodriguez-Galan MC, Pivetta M, Reynolds D, Howard OM, Palko ME, Esteban PF, Young HA, Rosato A, Tessarollo L.The scaffold protein Cybr is required for cytokine-modulated trafficking of leukocytes in vivo. Mol Cell Biol.2006;26:5249-58. PMCID:PMC1592701.
Esteban PF, Yoon HY, Becker J, Dorsey SG, Caprari P, Palko ME, Coppola V, Saragovi HU, Randazzo PA, Tessarollo L.A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin. J Cell Biol. 2006;173(2):291-9. PMCID:PMC2063819.
Coppola V, Barrick CA, Southon EA, Celeste A, Wang K, Chen B, Haddad el-B, Yin J, Nussenzweig A, Subramaniam A, Tessarollo L. Ablation of TrkA function in the immune system causes B cell abnormalities. Development. 2004;131(20):5185-95.
Tessarollo L., Coppola V., and Fritzsch B. NT-3 replacement with brain-derived neurotrophic factor redirects vestibular nerve fibers to the cochlea. J Neurosci. 2004; 24: 2575-84.
Sharan SK, Pyle A., Coppola V., Babus J., Swaminathan S., Benedict J., Swing D., Martin BK., Tessarollo L., Evans JP, Flaws JA., and Handel MA. BRCA2 deficiency in mice leads to meiotic impairment and infertility. Development, 2004; 131 131-142.
Wilson SM, Bhattacharyya B, Rachel RA, Coppola V, Tessarollo L, Householder DB, Fletcher CF, Miller RJ, Copeland NG, Jenkins NA. Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease. Nat Genet. 2002;32(3):420-5.
Celeste A, Petersen S, Romanienko PJ, Fernandez-Capetillo O, Chen HT, Sedelnikova OA, Reina-San-Martin B, Coppola V, Meffre E, Difilippantonio MJ, Redon C, Pilch DR, Olaru A, Eckhaus M, Camerini-Otero RD, Tessarollo L, Livak F, Manova K, Bonner WM, Nussenzweig MC, Nussenzweig A.Genomic instability in mice lacking histone H2AX. Science.2002;296:922-7.
Coppola V, Kucera J, Palko ME, Martinez-De Velasco J, Lyons WE, Fritzsch B, Tessarollo L. Dissection of NT3 functions in vivo by gene replacement strategy. Development. 2001;128:4315-27.
Coppola V, Veronesi A, Indraccolo S, Calderazzo F, Mion M, Minuzzo S, Esposito G, Mauro D, Silvestri B, Gallo P, Falagiani P, Amadori A, Chieco-Bianchi L. Lymphoproliferative disease in human peripheral blood mononuclear cell-injected SCID mice. IV. Differential activation of human Th1 and Th2 lymphocytes and influence of the atopic status on lymphoma development. J Immunol. 1998;160(5):2514-22.