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Freitas, Michael A.

Department of Cancer Biology and Genetics
Division of Human Cancer Genetics

Contact Information:

Office:

906 Biomedical Research Tower
460 W 12th Ave
Columbus, OH 43210
Ph. (614) 688-8432
E-Mail: michael.freitas@osumc.edu

Research Interests:

Our group has developed extensive expertise in the field of clinical/translational proteomics, biomarker discovery/validation and bioinformatics for mass spectrometry based proteomics. Our work focuses on the determination of side chain modifications of proteins important in Chromatin Remodeling and relating these modifications to aberrant cellular function in cancer.

Selected Publications:

Johnson EK, Zhang L, Adams ME, Phillips A, Freitas MA, Froehner SC, Green-Church KB, Montanaro F.Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins.PLoS One. 2012;7(8):e43515. Epub 2012 Aug 24.

Lapalombella R, Yeh YY, Wang L, Ramanunni A, Rafiq S, Jha S, Staubli J, Lucas DM, Mani R, Herman SE, Johnson AJ, Lozanski A, Andritsos L, Jones J, Flynn JM, Lannutti B, Thompson P, Algate P, Stromatt S, Jarjoura D, Mo X, Wang D, Chen CS, Lozanski G, Heerema NA, Tridandapani S, Freitas MA, Muthusamy N, Byrd JC. Tetraspanin CD37 directly mediates transduction of survival and apoptotic signals. Cancer Cell. 2012 May 15;21(5):694-708.

Sutton GM, Ptitsyn AA, Floyd ZE, Yu G, Wu X, Hamel K, Shah FS, Centanni A, Eilertsen K, Kheterpal I, Newman S, Leonardi C, Freitas MA, Bunnell BA, Gimble JM. Biological aging alters circadian mechanisms in murine adipose tissue depots. Age (Dordr). 2012 Mar 13. [Epub ahead of print]

Palamarchuk A, Yan PS, Zanesi N, Wang L, Rodrigues B, Murphy M, Balatti V, Bottoni A, Nazaryan N, Alder H, Rassenti L, Kipps TJ, Freitas M, Croce CM, Pekarsky Y. Tcl1 protein functions as an inhibitor of de novo DNA methylation in B-cell chronic lymphocytic leukemia (CLL). Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2555-60. Epub 2012 Jan 30.

Satoskar AA, Shapiro JP, Bott CN, Song H, Nadasdy GM, Brodsky SV, Hebert LA, Birmingham DJ, Nadasdy T, Freitas MA, Rovin BH. Characterization of glomerular diseases using proteomic analysis of laser capture microdissected glomeruli. Mod Pathol. 2012 May;25(5):709-21. doi:10.1038/modpathol.2011.205. Epub 2012 Jan 27.

You J, Wang L, Saji M, Olesik SV, Ringel MD, Lucas DM, Byrd JC, Freitas MA. High-sensitivity TFA-free LC-MS for profiling histones. Proteomics. 2011 Aug;11(16):3326-34. doi: 10.1002/pmic.201000445.

Peterson JM, Kline W, Canan BD, Ricca DJ, Kaspar B, Delfín DA, DiRienzo K, Clemens PR, Robbins PD, Baldwin AS, Flood P, Kaumaya P, Freitas M, Kornegay JN, Mendell JR, Rafael-Fortney JA, Guttridge DC, Janssen PM. Peptide-based inhibition of NF-κB rescues diaphragm muscle contractile dysfunction in a murine model of Duchenne muscular dystrophy. Mol Med. 2011 May-Jun;17(5-6):508-15. doi: 10.2119/molmed.2010.00263. Epub 2011 Jan 20.

You J, Wang L, Saji M, Olesik SV, Ringel MD, Lucas DM, Byrd JC, Freitas MA. High-sensitivity TFA-free LC-MS for profiling histones. Proteomics. 2011 Aug;11(16):3326-34. doi: 10.1002/pmic.201000445.

Wang L, Harshman SW, Liu S, Ren C, Xu H, Sallans L, Grever M, Byrd JC, Marcucci G, Freitas MA. Assaying pharmacodynamic endpoints with targeted therapy: flavopiridol and 17AAG induced dephosphorylation of histone H1.5 in acute myeloid leukemia. Proteomics. 2010 Dec;10(23):4281-92.

Xu H, Hsu PH, Zhang L, Tsai MD, Freitas MA. A dynamic noise level algorithm for spectral screening of peptide MS/MS spectra.BMC Bioinformatics. 2010 Aug 23;11:436. J Proteome Res. 2010 Jul 2;9(7):3384-93.

Lucas DM, Alinari L, West DA, Davis ME, Edwards RB, Johnson AJ, Blum KA, Hofmeister CC, Freitas MA, Parthun MR, Wang D, Lehman A, Zhang X, Jarjoura D, Kulp SK, Croce CM, Grever MR, Chen CS, Baiocchi RA, Byrd JC. The novel deacetylase inhibitor AR-42 demonstrates pre-clinical activity in B-cell malignancies in vitro and in vivo. PLoS One. 2010 Jun 3;5(6):e10941.

Yang L, Tu S, Ren C, Bulloch EM, Liao CL, Tsai MD, Freitas MA. Unambiguous determination of isobaric histone modifications by reversed-phase retention time and high-mass accuracy. Anal Biochem. 2010 Jan 1;396(1):13-22. Epub 2009 Aug 21.

Xu H, Freitas MA.  A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. BMC Bioinformatics. 2007 Apr 20;8:133.

Su X, Ren C, Freitas MA. Mass spectrometry-based strategies for characterization of histones and their post-translational modifications. Expert Rev Proteomics. 2007 Apr;4(2):211-25.

Tu S, Bulloch EM, Yang L, Ren C, Huang WC, Hsu PH, Chen CH, Liao CL, Yu HM, Lo WS, Freitas MA, Tsai MD. Identification of histone demethylases in Saccharomyces cerevisiae. J Biol Chem. 2007 May 11;282(19):14262-71.

Tsubota T, Berndsen CE, Erkmann JA, Smith CL, Yang L, Freitas MA, Denu JM, Kaufman PD. Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes. Mol Cell. 2007 Mar 9;25(5):703-12. Epub 2007 Feb 22.

Knapp AR, Ren C, Su X, Lucas DM, Byrd JC, Freitas MA, Parthun MR. Quantitative profiling of histone post-translational modifications by stable isotope labeling. Methods. 2007 Mar;41(3):312-9.

Su X, Zhang L, Lucas DM, Davis ME, Knapp AR, Green-Church KB, Marcucci G, Parthun MR, Byrd JC, Freitas MA. Histone H4 acetylation dynamics determined by stable isotope labeling with amino acids in cell culture and mass spectrometry. Anal Biochem. 2007 Apr 1;363(1):22-34.

Su X, Jacob NK, Amunugama R, Lucas DM, Knapp AR, Ren C, Davis ME, Marcucci G, Parthun MR, Byrd JC, Fishel R, Freitas MA. Liquid chromatography mass spectrometry profiling of histones. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):440-54.