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Freitas, Michael A., PhD

Associate Professor, Cancer Biology and Genetics
Division of Human Cancer Genetics
 
Director, CCC Proteomics Shared Resource

Contact Information:

Office:

906 Biomedical Research Tower
460 W 12th Ave
Columbus, OH 43210
Ph. (614) 688-8432
E-Mail: michael.freitas@osumc.edu

Research Interests:

Dr. Freitas is the Director of the OSU CCC Proteomics Shared Resource. He is an Associate Professor in the Department of Molecular Virology Immunology and Medical Genetics, and the Department of Biomedical Informatics in the College of Medicine.  He is a member of the OSUCCC Program.  Dr. Freitas has been working in the field of mass spectrometry for 27 years and has spent the last 18 years developing and improving mass spectrometry and bioinformatic methods for his research in the fields of cancer proteomics and chromatin biology. He is a recipient of the Camille and Henry Dreyfus New Faculty Award and the American Society for Mass Spectrometry Research Award. Dr. Freitas serves as a scientific advisor to the OSU Campus Chemical Instrument Center’s Mass Spectrometry and Proteomics Facility. Current research in the Freitas lab is focused on apply proteomic methods to understand chromatin regulatory networks in cancer.  The lab has extensive expertise in characterizing protein modifications and identifying protein:protein interactions by mass spectrometry. Selected Publications:

Agudelo Garcia PA, Hoover ME, Zhang P, Nagarajan P, Freitas MA, Parthun MR.
Identification of multiple roles for histone acetyltransferase 1 in replication-coupled chromatin assembly. Nucleic Acids Res. 2017 Jun 28. doi:
10.1093/nar/gkx545. [Epub ahead of print] PubMed PMID: 28666361.

Abbaoui B, Telu KH, Lucas CR, Thomas-Ahner JM, Schwartz SJ, Clinton SK, Freitas MA, Mortazavi A. The impact of cruciferous vegetable isothiocyanates on histone acetylation and histone phosphorylation in bladder cancer. J Proteomics.
2017 Mar 6;156:94-103. doi: 10.1016/j.jprot.2017.01.013. Epub 2017 Jan 27. PubMed
PMID: 28132875; PubMed Central PMCID: PMC5324139.

Wang L, Branson OE, Shilo K, Hitchcock CL, Freitas MA. Proteomic Signatures of Thymomas. PLoS One. 2016 Nov 10;11(11):e0166494. doi:
10.1371/journal.pone.0166494. eCollection 2016. PubMed PMID: 27832160; PubMed Central PMCID: PMC5104371.

Branson OE, Freitas MA. Tag-Count Analysis of Large-Scale Proteomic Data. J Proteome Res. 2016 Dec 2;15(12):4742-4746. Epub 2016 Nov 10. PubMed PMID:
27797532.

Zhang P, Branson OE, Freitas MA, Parthun MR. Identification of replication-dependent and replication-independent linker histone complexes: Tpr specifically promotes replication-dependent linker histone stability. BMC Biochem. 2016 Oct 1;17(1):18. PubMed PMID: 27716023; PubMed Central PMCID:
PMC5045598.

Branson OE, Freitas MA. A multi-model statistical approach for proteomic spectral count quantitation. J Proteomics. 2016 Jul 20;144:23-32. doi:
10.1016/j.jprot.2016.05.032. Epub 2016 May 31. PubMed PMID: 27260494; PubMed Central PMCID: PMC4967010.

Harshman SW, Canella A, Ciarlariello PD, Agarwal K, Branson OE, Rocci A, Cordero H, Phelps MA, Hade EM, Dubovsky JA, Palumbo A, Rosko A, Byrd JC, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F. Proteomic characterization of circulating extracellular vesicles identifies novel serum myeloma associated markers. J Proteomics. 2016 Mar 16;136:89-98. doi:
10.1016/j.jprot.2015.12.016. Epub 2016 Jan 13. PubMed PMID: 26775013; PubMed Central PMCID: PMC4783258.

Singh R, Harshman SW, Ruppert AS, Mortazavi A, Lucas DM, Thomas-Ahner JM, Clinton SK, Byrd JC, Freitas MA, Parthun MR. Proteomic profiling identifies specific histone species associated with leukemic and cancer cells. Clin Proteomics. 2015 Aug 27;12(1):22. doi: 10.1186/s12014-015-9095-4. eCollection 2015. PubMed PMID: 26321891; PubMed Central PMCID: PMC4551702.

Chen Y, Hoover ME, Dang X, Shomo AA, Guan X, Marshall AG, Freitas MA, Young NL. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics. 2016 Mar;15(3):818-33. doi:
10.1074/mcp.M114.046441. Epub 2015 Jul 24. PubMed PMID: 26209608; PubMed Central
PMCID: PMC4813703.

Harshman SW, Hoover ME, Huang C, Branson OE, Chaney SB, Cheney CM, Rosol TJ, Shapiro CL, Wysocki VH, Huebner K, Freitas MA. Histone H1 phosphorylation in breast cancer. J Proteome Res. 2014 May 2;13(5):2453-67. doi: 10.1021/pr401248f.
Epub 2014 Apr 7. PubMed PMID: 24601643; PubMed Central PMCID: PMC4012839.

Guan X, Rastogi N, Parthun MR, Freitas MA. SILAC peptide ratio calculator: a tool for SILAC quantitation of peptides and post-translational modifications. J Proteome Res. 2014 Feb 7;13(2):506-16. doi: 10.1021/pr400675n. Epub 2014 Jan 9.
PubMed PMID: 24328097; PubMed Central PMCID: PMC3953147.

Harshman SW, Chen MM, Branson OE, Jacob NK, Johnson AJ, Byrd JC, Freitas MA.
Isolation and analysis of linker histones across cellular compartments. J Proteomics. 2013 Oct 8;91:595-604. doi: 10.1016/j.jprot.2013.08.022. Epub 2013 Sep 5. PubMed PMID: 24013129; PubMed Central PMCID: PMC3863389.

Harshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM, Talabere T, Efebera YA, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F. Characterization of multiple myeloma vesicles by label-free relative quantitation. Proteomics. 2013 Oct;13(20):3013-29. doi: 10.1002/pmic.201300142.
PubMed PMID: 23983189; PubMed Central PMCID: PMC3835174.

Harshman SW, Young NL, Parthun MR, Freitas MA. H1 histones: current perspectives and challenges. Nucleic Acids Res. 2013 Nov;41(21):9593-609. doi:
10.1093/nar/gkt700. Epub 2013 Aug 14. Review. PubMed PMID: 23945933; PubMed Central PMCID: PMC3834806.

Telu KH, Abbaoui B, Thomas-Ahner JM, Zynger DL, Clinton SK, Freitas MA, Mortazavi A. Alterations of histone H1 phosphorylation during bladder carcinogenesis. J Proteome Res. 2013 Jul 5;12(7):3317-26. doi:
10.1021/pr400143x.
Epub 2013 Jun 17. PubMed PMID: 23675690; PubMed Central PMCID: PMC3743963.