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Associate Professor

Ludwig, Thomas, PhD

Department of Cancer Biology and Genetics

Contact Information

596 Biomedical Research Tower
460 West 12th Avenue, Columbus, OH 43210
Phone: (614) 685-5679
Fax: (614) 292-4118 

Research Description
The major focus of the Ludwig laboratory is to study the biological function(s) of BRCA1 and BRCA2 and some of their interacting partners in normal and malignant development. Using genetic engineering in mouse embryonic stem cells, we are generating genetically modified mice carrying knock-out, knock-in, and/or conditional alleles to elucidate the phenotypic consequences of specific mutations alone or in compound mutant mice during normal and tumor development.  In vivo tumor studies are complemented with in vitro assays (cell culture-based techniques on primary and tumor cells) and genomic approaches (expression profiling, aCGH, and sequencing) to elucidate the molecular mechanisms of disease progression. 



Recent Publications



1. Shakya, R., Gonda, TA., Quante, M., Salas, M., Kim, S., Brooks, J., Hirsch, S., Davies, J., Cullo, A., Olive, K.P., Szabolcs, M., Wang, T.C., Tycko, B., and Ludwig, T. (2013). Hypomethylating therapy in an  aggressive  stroma-rich  model  of  pancreatic  carcinoma.  Cancer  Res.  73,  885-896.  PMID:
2.  Bothmer,   A.,  Rommel,   P.C.,   Gazumyan,   A.,  Polato,   F.,  Reczek,   C.R.,   Muellenbeck,   M.F., Schaetzlein, S., Edelmann, W., Chen, P-L., Brosh, R.M., Casellas, R., Ludwig, T., Baer, R., Nussenzweig,  A., Nussenzweig,  M.C.,  and  Robbiani,  D.F. (2013).  Mechanism  of DNA  resection during  intrachromosomal  recombination  and  immunoglobulin  class  switching.  J. Exp.  Med.  210,
115-123. PMID: 23254285
3.  Gupta,  A.,  Hunt,  C.R.,  Pandita,  R.K.,  Pae,  J.,  Komal,  K.,  Singh,  M.,  Shay,  J.W.,  Kumar,  R., Hittelman, W.N., Guha, C., Ludwig, T. and Pandita, T.K. (2013). T-cell-specific  deletion of males absent on the first (Mof) blocks their differentiation and results in genomic instability. Mutagenesis
28, 263-270. PMID: 23386701
4.  González, F., Georgieva, D., Vanoli, F., Shi, Z.-D. Stadtfeld, M., Ludwig, T., Jasin, M., and Huangfu, D. (2013). Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state. Cell Rep. 3, 651-660. PMID: 23478019
5. Kass, E.M., Helgadottir, H.R., Chen, C.-C., Barbera, M., Wang, R., Westermark, U.K., Ludwig, T., Moynahan, M.E., and Jasin, M. (2013). Double-strand break repair by homologous recombination in primary mouse somatic cells: Requirement for BRCA1 but not the ATM kinase. Proc. Natl. Acad. Sci. USA 110, 5564-5569. PMID: 23509290
6. Lucas, A.L., Shakya, R., Lipsyc, M.D., Mitchel, E.B., Kumar, S., Hwang, C., Deng, L., Devoe, D., Chabot, J.A., Szabolcs,  M., Ludwig, T., Chung, W.K., and Frucht, H. (2013). High prevalence  of BRCA1  and  BRCA2  germline  mutations  with  loss  of  heterozygosity  in  a  series  of  resected pancreatic adenocarcinoma and other neoplastic lesions. Clin. Cancer Res. 19, 3396-3403. PMID:
7.  Reczek,  C.R.,  Szabolcs,  M., Stark,  J.M.,  Ludwig,  T.*, and  Baer,  R.* (2013).  *Co-Corresponding authors.  The  interaction  between  CtIP  and  BRCA1  is not essential  for resection-mediated  DNA repair or tumor suppression. J. Cell Biol. 201, 693-707. PMID: 23712259
8.  Hua, H., Shang, L., Martinez, H., Freeby, M., Gallagher, M.P., Ludwig, T., Deng, L., Greenberg, E., LeDuc, C., Chung, W.K., Goland, R., Leibel, R.L., and Egli, D. (2013). iPSC-derived β cells model diabetes due to glucokinase deficiency. J. Clin. Invest. 123, 3146-3153. PMID: 23778137
9.  Tian, F., Sharma, S., Zou,.J., Lin, S-Y., Wang, B., Rezvani, K., Wang, H., Parvin, J.D., Ludwig, T., Canman, C.E., and Zhang, D. (2013). BRCA1 promotes the ubiquitination of PCNA and recruitment of translesion polymerases in response to replication blockade. Proc. Natl. Acad. Sci. USA 110,
13558-13563. PMID: 23901102
10.  Xu, Y., Xu, Y., Liao, L., Zhou, N., Theissen, S., Liao, X-H., Nguyen, H., Ludwig, T., Qin, L., Jiang, J., and Xu, J. (2013). Inducible knockout of Twist1 in young and adult mice prolongs hair growth cycle and has mild effects on general health, supporting Twist1 as a preferential cancer target. Am. J. Pathol. 183, 1281-1292. PMID: 23906809
11.  Sonabend, A.M., Bansal, M., Guarnieri, P., Lei, L., Amendolara, B., Soderquist, C., Leung, R., Yun, J., Kennedy, B., Sisti, J., Bruce, S., Bruce, R., Shakya, R., Ludwig, T., Rosenfeld, S., Sims, P.A., Bruce, J.N., Califano, A., and Canoll, P. (2014). The transcriptional regulatory network of proneural glioma determines the genetic alterations selected during tumor progression. Cancer Res. 74, 1440-
1451. PMID: 24390738
12.  Polato, F., Callen, E., Wong, N., Faryabi, R., Bunting, S., Chen, H-T., Kozak, M., Kruhlak, M.J., Reczek, C.R., Lee, W-H., Ludwig, T., Baer, R., Feigenbaum, L., Jackson, S., and Nussenzweig, A. (2014). CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J. Exp. Med. 211, 1027-1036. PMID: 24842372
13. Gupta, A., Hunt, C.R., Hegde, M.L., Chakraborty, S., Udayakumar, D., Horikoshi, N., Singh, M., Ramnarain, D.B., Hittelman, W.N., Namjoshi, S., Asaithamby, A., Hazra, T.K., Ludwig, T., Pandita,
R.K., Tyler, J.K., and Pandita, T.K. (2014). MOF phosphorylation by ATM regulates 53BP1-mediated
DSB repair pathway choice. Cell Rep. 8, 177-189. PMID: 24953651
14.  Simhadri, S., Peterson, S., Patel, D., Le Masson, F., Huo, Y., Cai, H., Bowman-Colin, C., Miller, S., Barnard, N., Ludwig, T., Ganesan, S., Bhaumik, M., Bunting, S., Jasin, M., and Xia, B. (2014). Male fertility  defect  associated  with  disrupted  BRCA1-PALB2  interaction  in  mice.  J.  Biol.  Chem.  (in press). PMID: 25016020
15.  Shelef, M.A., Bennin, D.A., Yasmin, N., Warner, T.F., Ludwig, T., Beggs, H.E., and Huttenlocher, A. (2014).   Focal   adhesion   kinase   is  required   for  synovial   fibroblast   invasion,   but  not  murine inflammatory arthritis. Arthritis Res. Ther. (accepted for publication).