In the Human genome, well-studied sequences are protein-coding genes, which account for only 1.5–2% of the total genome; with the advancement in sequencing technologies now, it is evident that a significant proportion of them are non-coding RNAs (ncRNAs). We evaluate the biogenesis and function of short ncRNAs micro RNA (miRNA), small nucleolar RNA (snoRNA) and Piwi-interacting RNA (), and long ncRNAs linear long-noncoding RNA (lncRNA) and circular RNA () in cardiovascular health and disease. We employ various modern and classical technologies such as RNA sequencing, single-molecule FISH, non-coding RNA pull-down assays, exosomes isolation, AAV9 mediated non-coding RNA delivery, LNA based non-coding RNA silencing, and mouse models (diabetic and non-diabetic) of myocardial infarction. We believe identifying and characterizing non-coding RNA that play a regulatory role in the network of interactions governing cardiovascular health and disease is a real puzzle that needs to be solved to allow better treatment and prevention of cardiovascular disease. Our long-term goal is to identify novel therapeutic targets for the treatment of myocardial infarction.
lab focuses on the following research areas:
- Role of long ncRNA (lncRNA and circular RNA) in diabetic cardiomyopathy
- Role of small ncRNA (miRNA, , snoRNAs, and ) in cardiovascular health and disease
- Role of exosomal- ncRNA (lncRNA, circular RNA, miRNA, snoRNAs, and ) in cardiovascular health and disease
- We are also investigating the space flight associated exosomal RNA changes in the astronaut's plasma (In collaboration with at Mount Sinai, NY).
Venkata Naga Srikanth Garikipati, PhD
Amit Kumar Rai, PhD
Suriya Muthukumaran Natarajaseenivasan, PhD
Brooke Lee, BS