Substitutions in 18 bp or more bases at a target site by a single round reaction

Insertion of 18 bp or more bases at a target site by a single round reaction

Deletion of any short or long target sequence by a single round reaction

Chimeragenesis: Generation of chimeric genes by combining several gene fragments

Multiple-site mutagenesis: Introduce any kinds of mutations at two or more separate sites simultaneously by a single round reaction

Random mutagenesis: Construction of mutant library with a high diversity by error-prone PCR or site-specific random mutagenesis (site saturation)

Gene subcloning and synthesis: PCR subcloning of the gene of interest in customer's vector with or without tag sequences (6xHis, myc, HA, etc.)

Customized vector construction: Construction of any complicate vector system according to customer's request

Construction of expression vector for 2nd generation shRNA (a short-hairpin RNA)
Prediction of efficient target sites and design expression vector
Synthesis and subcloning of shRNA oligos to construct an shRNA expression vector

Construction of gRNA/Cas9 expression vector for CRISPR-mediated gene knock-out
Prediction of efficient and specific gRNA target sites and design gRNA/Cas-9 expression vector
Construction of gRNA/Cas9 vector according to customer's CRISPR strategy

• Before placing an order, please contact us for pricing information. You can easily request a quotation by email. To estimate an exact quote and turnaround term, we need information for your gene, construct and target mutations. For your convenience, please download and fill out a related quote request form linked below. We will provide a quote in 24 hours.
• To start a mutagenesis project, we need your signed order form, wt construct containing your target gene and construct information such as sequence and map.
• We will start the mutagenesis reaction as soon as we receive your ordering package and your gene sample.

Step 1.Get a quotation: e-mail, FAX or by phone

Step 2. Submission of ordering package:

• Order form: by e-mail or a regular mail of a signed hard copy
• Target gene sample (in a plasmid or other vectors)
• Information of gene and construct (vector)

For customers in the Ohio State University and the Wexner Medical center, please place an order with the submission of your quote via eRAMP.

Step 3. Mutagenesis and confirmation of a target mutation by the MBC lab

Step 4. Delivery of purified mutant construct, sequencing data, and mutagenesis report

• Quote request form, (PDF), (Word Document)
• Order form, (PDF), (Word Document)

If you are a researcher in the Ohio State University and the Wexner Medical Center, please place an order through eRAMP with the submission of your quote.

eRAMP