Dr. Dan Wozniak received an R01 Grant for $1,389,063 from the National Institute of Health and NIAID for research on “Pseudomonas Biofilms and Immunity.”Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes chronic lung infections, which are predominantly associated with biofilm formation in the airways of patients with cystic fibrosis (CF) and immune compromised patients, including those with chronic wounds. Biofilms are structured communities of microbes encased within a matrix and exhibit resistance to antimicrobials and host defenses. The research will focus on investigating structure-function relationships necessary for P. aeruginosa biofilm matrix integrity by defining the roles of two crucial components of the P. aeruginosa biofilm matrix, an extracellular polysaccharide Psl and a protein adhesion CdrA, specifically how these components modulate the interactions of P. aeruginosa with human phagocytic cells. Furthermore, Dr. Wozniak will investigate a novel signal transduction pathway responsible for a feed-forward mechanism of Psl-dependent-signaling. The research will also show how one component of the biofilm matrix serves as a signaling molecule to communicate with other members of the biofilm community. The ultimate goal of the research is to enhance the interaction of host immune cells and biofilms of P. aeruginosa so that biofilms can be eradicated from the lungs of (CF) patients as well as from individuals with other P. aeruginosa infections.