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Dr. Sashwati Roy's Lab image on wound inflammation has been selected for the cover of the June 15, 2016 issue of The Journal of Immunology. 

 

Dr. Sashwati Roy's Lab image on wound inflammation has been selected for the cover of the June 15, 2016 issue of The Journal of Immunology.


Diabetic wounds suffer from persistent inflammation due to the burden of dead cells at the wound site. Studies from the lab of Dr. Sashwati Roy has identified MFG-E8, a key protein involved in the removal of apoptotic cells, to be glycated in the hyperglycemic wound milieu. Topical treatment with recombinant MFG-E8 resolved diabetic wound inflammation by facilitating apoptotic cell clearance which positively promoted wound healing. Details of the sudy may be obtained from:

 Ref: Das A, Ghatak S, Sinha M, Chaffee S, Ahmed NS, Parinandi NL, Wohleb ES, Sheridan JF, Sen CK, Roy S. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes. J Immunol. 2016 Jun 15;196(12):5089-100. doi: 10.4049/jimmunol.1502270. Epub 2016 May 18.


Scientific Abstract

Milk fat globule epidermal growth factor-factor 8 (MFG-E8) is a peripheral glycoprotein that acts as a bridging molecule between the macrophage and apoptotic cells, thus executing a pivotal role in the scavenging of apoptotic cells from affected tissue. We have previously reported that apoptotic cell clearance activity or efferocytosis is compromised in diabetic wound macrophages. In this work, we test the hypothesis that MFG-E8 helps resolve inflammation, supports angiogenesis, and accelerates wound closure. MFG-E8−/− mice displayed impaired efferocytosis associated with exaggerated inflammatory response, poor angiogenesis, and wound closure. Wound macrophage-derived MFG-E8 was recognized as a critical driver of wound angiogenesis. Transplantation of MFG-E8−/− bone marrow to MFG-E8+/+ mice resulted in impaired wound closure and compromised wound vascularization. In contrast, MFG-E8−/− mice that received wild-type bone marrow showed improved wound closure and improved wound vascularization. Hyperglycemia and exposure to advanced glycated end products inactivated MFG-E8, recognizing a key mechanism that complicates diabetic wound healing. Diabetic db/db mice suffered from impaired efferocytosis accompanied with persistent inflammation and slow wound closure. Topical recombinant MFG-E8 induced resolution of wound inflammation, improvements in angiogenesis, and acceleration of closure, upholding the potential of MFG-E8–directed therapeutics in diabetic wound care. Ref: Das A, Ghatak S, Sinha M, Chaffee S, Ahmed NS, Parinandi NL, Wohleb ES, Sheridan JF, Sen CK, Roy S. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes. J Immunol. 2016 Jun 15;196(12):5089-100. doi: 10.4049/jimmunol.1502270. Epub 2016 May 18.

 

Posted on 7-Jun-16 by Toto, Brent
Tags: OSU Regenerative Medicine News
 
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