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Daniel Wozniak, PhD

Daniel Wozniak, PhD
Professor, Microbial Infection and Immunity Microbiology
Center for Microbial Interface Biology, The Ohio State University
Phone:  (614) 247-7629
Email: daniel.wozniak@osumc.edu

Dr. Wozniak’s research activities are focused on the pathogenesis of several bacteria that cause chronic, devastating infections in humans. In chronic wounds. Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter are the most common nosocomial pathogens isolated and are consistently associated with high mortality rates. Resources spent treating such infections in the U.S. are estimated at ~ $25 billion annually. These infections are extremely difficult to control since the bacteria exhibit a biofilm-mode of growth rendering them resistant to antimicrobials and phagocytic cells. Dr. Wozniak brings 25 years of experience in the area of microbial genetics, biofilms, polysaccharide gene regulation, and pathogenesis.
Team Members
Sheri Dellos-Nolan, B.S., Lab manager
Sarah Chaney, D.V.M, Graduate student
Dominique Limoli, B.S., Graduate student
Binjie Xu, B.S., Graduate student
Meenu Mishra, Ph.D., Postdoctoral fellow
Chris Jones, M.S., Graduate student
Ethan Mann, Ph.D., Postdoctoral fellow
Preston Hill, B.S., Research technician
Research Interests
Coordinate regulation of the virulence factors that contribute to P. aeruginosa pathogenesis.
Role of biofilm matrix components in promoting recalcitrance to host derived antimicrobials and cells
Experimental therapeutics for combating persistent biofilm infections.
Defining pathoadaptive processes and evolution of bacteria during infection.
Selected Publications
E. Mann and D.J. Wozniak.  2012.  Pseudomonas biofilm matrix composition and function.  FEMS Microbiol. Rev. In Press.

M. Mishra, M.S. Byrd, S. Sergeant, A. Azad, M.R. Parsek, L. McPhail, L.S. Schlesinger, and D.J. Wozniak.  2012.  Pseudomonas aeruginosa Psl polysaccharide reduces neutrophil phagocytosis and the oxidative response by limiting complement-mediated opsonization.  Cellular Micro.  14:95-106.

K. Colvin, V. Gordon, K. Murakami, B. R. Borlee, D.J. Wozniak, G. Wong, and M.R. Parsek.  2011.  The Pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa. PLoS Pathogens, 7(1): e10001264. Doi:10.1371/journal.ppat.10001264.

E.A. Waligora, D.M. Ramsey, E.E. Pryor, H. Lu, G.P. Sloan, T. Hollis, R. Deora, and D.J.Wozniak.  2010. AmrZ beta-sheet residues are essential for DNA-binding and transcriptional control of Pseudomonas aeruginosa virulence genes.  J. Bacteriol., 192:5390-5401.

M. S. Byrd, B. Pang, M. Mishra, W.E. Swords, and D.J. Wozniak.  2010. The Pseudomonas aeruginosa exopolysaccharide Psl facilitates surface adherence and NF-κB activation in A549 cells.  mBio.  1(2):e00140-10.doi10.1128/mBio.00140-10.

M. S. Byrd, I. Sadovskaya, E. Vinogradov, H. Lu, A. B. Sprinkle, S. H. Richardson, L. Ma, B. Ralston, M. R. Parsek, E. M. Anderson, J. S. Lam, and D. J. Wozniak. 2009.  Genetic and biochemical analyses of the Pseudomonas aeruginosa Pslexopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.  Mol. Microbiol. 73:622-638.

Luyan Ma, M. Conover, H. Lu, M. R. Parsek, K. Bayles, and D.J. Wozniak.  2009. Assembly and development of the Pseudomonas aeruginosa biofilm matrix.  PLoS Pathogens.  5:e1000354.