Our Facilities

Our laboratory is well-equipped with extensive facilities for molecular biology, transplantation surgery and ex-vivo perfusion. COPPER Laboratory offers services and resources in molecular biology and collaborates with ULAR. The lab is comprised of a small animal surgery suite, a large animal non-survival operating room, an organ perfusion suite and an organ and tissue processing center. COPPER Laboratory is located in Wiseman Hall.

Tissue culture hood

Areas of Expertise

Cell culture and molecular biology

Molecular Biology and Cell Culture

  • POLARstar Omega microplate reader with atmospheric control system and automated kinetic control modules. Read capability includes full UV/vis spectrum, luminescence and fluorescence intensity/resonance/polarization.
  • ThermoFisher iBright FL1500 gel & blot imaging documentation system with 9.1 MP camera. Capable of five read modes enabling colorimetry, densitometry, chemiluminescence, and UV. Fluorescence imaging of up to 4-color fluorescent western blot multiplexing.
  • Caron's Oasis™ CO₂ water-jacketed incubators for CO2 controlled cell culture in hypoxic or normoxic conditions.
  • Cell culture class II biological safety cabinet
  • ThermoFisher Countess3 FL automated cell counters,
  • BioRad PowerPac electrophoresis power supplies
  • SimpliAMP Thermal Cycler
  • QuantStudio 3 Real-Time PCR System
  • NanoDrop DNA/RNA/Protein quantitation spectrophotometer
  • LFX BioTEK- Lionheart FX Automated Live Cell Imager
  • Leica Thunder Microscope 3D imaging system equipped with both a Leica K5 fluorescence imaging camera and a Leica K3 color imaging camera
  • Lab equipment for cell culture (including scales, temperature controlled benchtop and swinging bucket centrifuges, water bath, pH meter, inverted light microscope etc.). Cryogenic storage including -80°C freezer, -20°C freezers, 4°C refrigerators, and liquid nitrogen storage within the laboratory space.
  • Alabama R&D precision cut tissue slicer enabling precise micrometer-resolution sections of three-dimensional animal and human samples.
Scientist at work in the lab

Small Animal

  • flexiVent Complete System Solution, SCIREQ Scientific
  • flexiVent FX Module 4 Package, SCIREQ Scientific
  • flexiVent FEV extension for rats, SCIREQ Scientific
  • Mouse vivoFlow plethysmography chambers, SCIREQ Scientific
  • Whole-body plethysmography chambers for rats, SCIREQ Scientific
  • ETI-MSE, Mouse Endotracheal Intubation Kit, Kent Scientific
  • ETI-RAT, Rat Endotracheal Intubation Kit, Kent Scientific
  • Ex vivo rat lung perfusion circuit (Harvard Apparatus IL-2)
  • Werfen iGEM5000 Blood Gas Analyzer
  • Prescott’s Inc. Leica M500 operative microscope
  • Ex vivo lung large animal perfusion circuit
  • Pentax flexible bronchoscope
  • Bio-Medicus Bio-Console Blood Pump centrifugal perfusion pump
  • Harvard Apparatus 683 small animal ventilator
  • Kent Scientific 668 Somnosuite small animal ventilator
  • Siemens 300 Servo clinical / large animal ventilator
  • Abaxis VetscanVS2
  • Gonotec Osmomat 010 Freezing point osmometer
  • Gonotec Osmomat 050 Colloid osmometer
Large Animal

Large Animal

  • Ex vivo lung large animal perfusion circuit
  • Ex vivo liver large animal perfusion circuit
  • Large animal anesthesia system
  • Echocardiography and ultrasonography
  • Fluoroscopy C-Arm
  • Bronchoscopy
  • Blood gas and serum analyzers
Lab scientist working at the computer

Extensive Lab Support Structure

  • Computers
  • Flatbed scanners
  • -80 freezer
  • -20 freezer
  • Refrigeration
  • Liquid nitrogen storage
Model systems

Model Systems

  • Cell culture
  • Primary and immortalized cell lines
    • Human umbilical vein endothelial cell (HUVEC)
    • Human pulmonary artery endothelial cell (HPAEC)
    • Human aortic endothelial cell (HAoEC)
    • Porcine pulmonary artery endothelial cell (PPAEC)
    • Porcine aortic endothelial cell line (PAoEC)
    • Human alveolar basal epithelial cells (A549)
    • Hepatocyte cells (HepG2)
  • Rat
  • Pig
  • Sheep
  • Cadaveric