Narasimham Parinandi, PhD

Associate Professor, Division of Pulmonary, Critical Care and Sleep Medicine

Narasimham Parinandi

Associate Professor
Research & Teaching

5180-Graves Hall & 5139-Optometry Clinic, OSU Wexner Medical Center
5130-Graves Hall, OSU Wexner Medical Center
narasimham.parinandi@osumc.edu; Parinandi.3@osu.edu
(614) 292-8577 Narasimham.Parinandi@osumc.edu

PubMed Articles Google Scholar ResearchGate

Teaching, Mentoring, and Research Interests

Teaching & Mentoring: Teaches wide variety of topics in Life Sciences and Biochemistry and Environmental Sciences. Passionate and committed to mentor undergraduate and graduate students on independent projects in fundamental research in Biological Sciences and Biochemistry.

Research: Biochemistry & Toxicology; Lipidomics of Phospholipases and Bioactive Lipids; Lipid Signaling in Regulation of Secretory Phenomena; Heavy Metal and Lipid Bilayer Interactions; Oxidized Lipidome; Translational Lipidomics of Chronic Diseases; Analytical Biochemistry; Methods of Teaching and Mentoring Research.

Research Interests

Oxidant Lipid Signaling; Vascular Biology; Phospholipases A2 and D; Analytical Lipidomics; Oxidative Stress; Bioinorganic Chemistry

Current Research:
Phospholipase A2 and D regulation in Lung Vascular Endothelium and Epithelium; Membrane Lipid Dynamics; Oxidative Lipidomics of Eicosanoids and Oxidized PUFA; Redox Antioxidant Biochemistry in Lung Damage; Heavy Metal Toxicology

Active Funding

  1. R01: 64000015; (PI: Jodi McDaniel); Role: Co-I (Parinandi: 10% FTE)
    NIH/National Institute on Aging
    09/30/2018 - 03/31/2024
    Total amount awarded: $ 3,331,920.00
    Impact of Omega-3 Fatty Acid Oral Therapy on Healing of Chronic Venous Leg Ulcers in Older Adults.
    This project investigates the role of omega-3 fatty acids in healing of wounds in human subjects.
  2. R01: 1 R01 AI154553-01 (PI: Amal Amer); Role: Co-I (Parinandi: 5% effort)
    NIH/MPI
    Award period: from 12/01/2020 to 11/30/2025
    Total amount awarded: $ 3,030,911.00
    The Inflammasome and host-Legionella interaction.
    The goal of this project and my role is to investigate the cytoskeletal rearrangement and the ECIS behavior of the macrophages under Legionella infection.

Education and Training

  • University of Berhampur, India B.Sc.(Hons) 1971-75 Chem/Botany/Zoology/English Literature
  • University of Berhampur, India M.Sc. 1975-77 Botany – Environ. Biol.
  • University of Andhra, India Post-Grad 1977-80 Environmental Sciences
  • University of Toledo, Toledo, OH Ph.D. 1980-86 Biology - Toxicology
  • University of Minnesota, Hormel Institute, MN Post-Doc 1986-90 Lipid Biochemistry
  • Johns Hopkins University, Baltimore, MD Res Assoc1998-02 Oxidant Lipid Signaling

Honors and Awards

  • 2009 Distinguished Undergraduate Research Mentor, The Oho State University Denman Undergraduate Research Forum, Columbus, OH
  • 2008 Distinguished Mentor Award, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH
  • 2012 U.S. Environmental Protection Agency (EPA) 2012 Honorable Mention in Scientific & Technological Achievement Award Competition for Research Publication, “Vascular and Cardiac Impairments in Rats Inhaling Ozone and Diesel Exhaust Particles” in Environmental Health Perspectives.
  • 1998 Recognition by the American Chemical Society (ACS), Columbus Chapter, Columbus, OH
  • 1997 Recognition by the Chemical Abstracts Service for launching SciFinder, Columbus, OH
  • 1986 Outstanding Teaching Assistant Award, Department of Biology, University of Toledo, OH
  • 1977 University Gold Medal for scoring highest GPA in the M.S. program, University of Berhampur, India
  • 1975-77 National Merit Scholarship, Govt. of India, University of Berhampur, India

Select Publications

(list of high impact pubs out of 130)

  1. Parinandi, N.L. and Schmid, H.H.O. (1988). Effects of long-chain N-acylethanolamines on lipid peroxidation in cardiac mitochondria. FEBS Lett.237(1-2):49-52.
  2. Parinandi, N.L., Weis, B.K., and Schmid, H.H.O. (1988). Assay of cardiolipin peroxidation by high-performance liquid chromatography. Chem. Phys. Lipids 49(3):215-220.
  3. Parinandi, N.L., Weis, B.K., Natarajan, V., and Schmid, H.H.O. (1990). Peroxidative modification of phospholipids in myocardial membranes. Arch. Biochem. Biophys.280(1):45-52.
  4. Parinandi, N.L., Thompson, Ed.W., and Schmid, H.H.O. (1990). Diabetic heart and kidney exhibit increased resistance to lipid peroxidation. Biochem. Biophys. Acta1047(1):63-69.
  5. Parinandi, N.L., Zwizinski, C.W., and Schmid, H.H.O. (1991). Free radical-induced alterations of myocardial membrane proteins. Arch. Biochem. Biophys.289(1):118-123.
  6. Natarajan, V., Mohiuddin, M., Roehm, B., Parinandi, N.L., Schmid, H.H.O., Kiss, Z., and Garcia, J.G.N. (1993). Activation of endothelial cell phospholipase D by hydrogen peroxide and fatty acid hydroperoxide. J. Biol. Chem.268(2):930-937.
  7. Parinandi, N.L., Scribner, W.M., Vepa, S., Shi, S., and Natarajan, V. (1999). Phospholipase D activation in endothelial cells is redox sensitive. Antioxidants & Redox Signaling1(2):193-210.
  8. Shi, S., Garcia, J.G.N., Roy, S., Parinandi, N.L., and Natarajan, V. (2000). Involvement of c-Src in diperoxovanadate-induced endothelial barrier dysfunction. Am. J. Physiol. Lung Cell Mol. Physiol. 279:L441-L451.(Editorial Focus Paper).
  9. Parinandi, N.L., Roy, S., Cummings, R., Morris, A.J., Garcia, J.G.N., and Natarajan, V. (2001). Role of Src kinase in diperoxovanadate-mediated activation of phospholipase D in endothelial cells. Arch. Biochem. Biophys. 396(2), 231-243.
  10. Cummings, R., Parinandi, N.L., Zaiman, A., Wang, L., Garcia, J.G.N., and Natarajan, V. (2001): Phospholipase D activation by sphingosine-1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells. J. Biol. Chem.16;277(33):30227-35, 2002.
  11. Varadharaj S, Watkins T, Cardounel AJ, Joe G.N. Garcia, Zweier JL, Kuppusamy, P., Natarajan V, Parinandi NL. (2005). Vitamin C-induced loss of redox-dependent viability in lung microvascular endothelial cells. Antioxidants Redox Signal. 7(1-2):287-300.
  12. Varadharaj, S., Sakthivel, R., Vedamoorthyrao, S.,Parinandi, N.L. (2005). Alterations in band 3 protein and anion exchange in red blood cells of renal failure patients. J. Mol. Cell. Biochem.2005 May; 273(1-2):11-24.
  13. Varadharaj S, Steinhour E, Hunter M, Watkins T, Baran C, Magalang U, Kuppusamy P, Marsh C, Zweier JL, Natarajan V, Parinandi NL. (2006) Vitamin C-induced activation of phospholipase D in lung endothelial cells: Regulation by MAP kinases. Cell Signaling. 2006 18(9):1396-407.
  14. Bratasz A, Weir NM, Parinandi NL, Zweier JL, Sridhar R, Ignarro LJ, Kuppusamy P. (2006) Reversal to cisplatin sensitivity in recurrent human ovarian cancer cells by NCX-4016, a nitro derivative of aspirin. Proc Natl Acad Sci U S A. 2006, 7;103(10):3914-9.(With Nobel Laureate, Dr. Ignarro).
  15. Usatyuk PV, Parinandi NL, Natarajan V. (2006) Redox regulation of 4-HNE-mediated endothelial barrier dysfunction by focal adhesion, adherens and tight junction proteins. J Biol Chem. 2006 Nov 17;281(46):35554-66.
  16. Parinandi NL, Sharma A, Eubank TD, Kaufman BF, Kutala VK, Marsh CB, Ignarro LJ, Kuppusamy P. (2007). Nitroaspirin, NCX-4016, is antiangiogenic through induction of loss of redox-dependent viability and cytoskeletal reorganization in endothelial cells. Antioxid Redox Signal. 2007 Nov;9(11):1837-49. (With Nobel Laureate, Dr. Ignarro).
  17. Hagele TJ, Mazerik JN, Gregory A, Kaufman BF, Magalang U, Kuppusamy M, Marsh CB, Kuppusamy P, Parinandi NL. (2007). Mercury activates vascular endothelial cell phospholipase D through thiols and oxidative stress. Int. J. Toxicol.2007 Jan-Feb;26(1):57-69.
  18. Usatyuk PV, Romer L, He D, Parinandi NL, Kleinberg ME, Zhan S, Jacobson JR, Dudek S, Garcia JGN, Natarajan V. (2007). Regulation of hyperoxia-induced NADPH oxidase activation in human lung endothelial cells by the actin cytoskeleton and cortactin. J Biol Chem. 2007 Aug 10;282(32):23284-95.
  19. Mazerik JN, Mikkilineni H, Kuppusamy VA, Steinhour E, Peltz A, Marsh CB, Kuppusamy P, Parinandi NL. (2007). Mercury activates phospholipase A2 and induces formation of arachidonic acid metabolites in vascular endothelial cells. Toxicol. Mech. Methods. 2007, 17: 541-557.
  20. Mazerik JN, Hagele T, Sherwani S, Ciapala V, Butler S, Kuppusamy ML, Hunter M, Kuppusamy P, Marsh CB, Parinandi NL. (2007). Phospholipase A2 activation regulates cytotoxicity of methylmercury in vascular endothelial cells. Int J Toxicol. 2007 Nov-Dec;26(6):553-69.
  21. Steinhour E, Sherwani S, Mazerik JN, Ciapala V, Butler S, Cruff JP, Magalang U, Parthasarathy S, Sen CK, Marsh CB, Kuppusamy P, Parinandi NL. (2008). Redox-active antioxidant modulation of lipid signaling in vascular endothelial cells: Vitamin C induces activation of phospholipase D through phospholipase A2, lipoxygenase, and cyclooxygenase. Mol Cell Biochem. 2008 Aug;315(1-2):97-112.
  22. Sathishkumar K, Gao X, Raghavamenon AC, Parinandi NL, Pryor WA, Uppu RM. (2009). Cholesterol secoaldehyde induces apoptosis in H9c2 cardiomyoblasts through reactive oxygen species involving mitochondrial and death receptor pathways. Free Radic Biol Med. 2009 Sep 1;47(5):548-58.
  23. Peltz A, Sherwani SI, Kotha SR, Mazerik JN, O'Connor Butler ES, Kuppusamy ML, Hagele T, Magalang UJ, Kuppusamy P, Marsh CB, Parinandi NL. (2009). Calcium and calmodulin regulate mercury-induced phospholipase D activation in vascular endothelial cells. Int J Toxicol. 2009 May-Jun;28(3):190-206.
  24. Sliman SM, Eubank TD, Kotha SR, Kuppusamy ML, Sherwani SI, Butler ES, Kuppusamy P, Roy S, Marsh CB, Stern DM, Parinandi NL. (2009). Hyperglycemic oxoaldehyde, glyoxal, causes barrier dysfunction, cytoskeletal alterations, and inhibition of angiogenesis in vascular endothelial cells: aminoguanidine protection. Mol Cell Biochem. 2010 Jan;333(1-2):9-26.
  25. Patel RB, Kotha SR, Sherwani SI, Sliman SM, Gurney TO, Loar B, O’Connor Butler S, Morris AJ, Marsh CB, Parinandi NL. (2011). Pulmonary fibrosis inducer, bleomycin, causes redox-sensitive activation of phospholipase D and cytotoxicity through formation of bioactive lipid signal mediator, phosphatidic acid, in lung microvascular endothelial cells. Int J Toxicol. 2011 Feb;30(1):69-90.
  26. Kodavanti UP, Thomas R, Ledbetter AD, Schladweiler MC, Shannahan JH, Wallenborn JG, Lund AK, Campen MJ, Butler EO, Gottipolu RR, Nyska A, Richards JE, Andrews D, Jaskot RH, McKee J, Kotha SR, Patel RB, Parinandi NL. (2011). Vascular and cardiac impairments in rats inhaling ozone and diesel exhaust particles. Environ Health Perspect. 2011 Mar;119(3):312-8.
  27. Crouser ED, Parinandi N. (2011). Mitochondrial mechanisms are at the 'heart' of novel ischemia-reperfusion therapies. Crit Care Med. 2011 Mar;39(3):593-5.
  28. Secor JD, Kotha SR, Gurney TO, Patel RB, Kefauver NR, Gupta N, Morris AJ, Haley BE, Parinandi NL. (2011). Novel lipid-soluble thiol-redox antioxidant and heavy metal chelator, N,N'-bis(2-mercaptoethyl)isophthalamide (NBMI) and phospholipase D-specific inhibitor, 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) attenuate mercury-induced lipid signaling leading to protection against cytotoxicity in aortic endothelial cells. Int J Toxicol. 2011 Dec;30(6):619-38.
  29. Patel RB, Kotha SR, Sauers LA, Malireddy S, Gurney TO, Gupta NN, Elton TS, Magalang UJ, Marsh CB, Haley BE, Parinandi NL. (2012). Thiol-redox antioxidants protect against lung vascular endothelial cytoskeletal alterations caused by pulmonary fibrosis inducer, bleomycin: comparison between classical thiol-protectant, N-acetyl-L-cysteine, and novel thiol antioxidant, N,N'-bis-2-mercaptoethyl isophthalamide. Toxicol Mech Methods. 2012 Jun;22(5):383-96.
  30. Parinandi NL, Magalang UJ. (2013). Avatars of adipose tissue: the saga of transformation of white fat, the villain into brown fat, the protector. Focus on "Inflammation induced by RAW macrophages suppresses the UCP1 mRNA induction via ERK activation in 10T1/2 adipocytes". Am J Physiol Cell Physiol. 2013 Apr 15;304(8):C715-6.
  31. Sherwani SI, Pabon S, Patel RB, Muzzammil MS, Hagele T, Kotha SR, Magalang UJ, Maddipati KR and Parinandi NL. (2013). Eicosanoid signaling of methylmercury-induced phospholipase D activation in vascular endothelial cells: Bioactive lipid-mediated cytotoxicity of methylmercury. Cell Biochem Biophys. 2013 Nov;67(2):317-29.
  32. Kotha SR, Piper MG, Patel RB, Sliman S, Malireddy S, Baran CP, Nana-Sinkam SP, Wewers MD, Romberger D, Marsh CB, Parinandi NL. (2013). Phospholipase A2 activation by poultry particulate matter is mediated through extracellular signal-regulated kinase in lung epithelial cells: Regulation of interleukin-8 release. Cell Biochem Biophys. 2013 Nov;67(2):415-29.
  33. Sliman SM, Patel RB, Cruff JP, Kotha SR, Newland CA, Schrader CA, Sherwani SI, Gurney TO, Magalang UJ, Parinandi NL. (2013). Adiponectin protects against hyperoxic lung injury and vascular leak. Cell Biochem Biophys. 2013 Nov;67(2):399-414.
  34. Huang LS, Mathew B, Li H, Zhao Y, Ma SF, Noth I, Reddy SP, Harijith A, Usatyuk PV, Berdyshev EV, Kaminski N, Zhou T, Zhang W, Zhang Y, Rehman J, Kotha SR, Gurney TO, Parinandi NL, Lussier YA, Garcia JG, Natarajan V. (2014). The mitochondrial cardiolipin remodeling enzyme lysocardiolipin acyltransferase is a novel target in pulmonary fibrosis. Am J Respir Crit Care Med. 2014 Jun 1;189(11):1402-15.
  35. Parinandi NL, Maulik N, Thirunavukkarasu M, McFadden D. Antioxidants in longevity and medicine. Oxidative Medicine and Cellular Longevity, 2015.
  36. Heisler DB, Kudryashova E, Grinevich DO, Suarez C, Winkelman JD, Birukov KG, Kotha SR, Parinandi NL, Vavylonis D, Kovar DR, Kudryashov DS. ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization. Science. 2015 Jul 31;349(6247):535-9.
  37. Das A, Ghatak S, Sinha M, Chaffee S, Ahmed NS, Parinandi NL, Wohleb ES, Sheridan JF, Sen CK, Roy S. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes. J Immunol. 2016 Jun 15;196(12):5089-100.
  38. McDaniel JC, Szalacha L, Sales M, Roy S, Chafee S, Parinandi N. EPA + DHA supplementation reduces PMN activation in microenvironment of chronic venous leg ulcers: A randomized, double-blind, controlled study. Wound Repair Regen. 2017 Aug;25(4):680-690.
  39. Oghumu S, Varikuti S, Saljoughian N, Terrazas C, Huntsman AC, Parinandi NL, Fuchs JR, Kinghorn AD, Satoskar AR. Pentalinonsterol, a Constituent of Pentalinon andrieuxii, Possesses Potent Immunomodulatory Activity and Primes T Cell Immune Responses. J Nat Prod. 2017 Sep 22;80(9):2515-2523.
  40. Kulkarni A, Kuppusamy P, Parinandi NL. (2007). Oxygen: The lead actor in the pathophysiological drama: Enactment of the trinity of normoxia, hypoxia, and hyperoxia in disease and therapy. Antioxid Redox Signal. 2007 Oct;9(10):1717-30.
  41. Malireddy S, Kotha SR, Secor JD, Gurney TO, Abbott JL, Maulik G, Maddipati KR, Parinandi NL. (2012). Phytochemical antioxidants modulate mammalian cellular epigenome: implications in health and disease. Antioxid Redox Signal. 2012 Jul 15;17(2):327-39.
  42. Elton TS, Selemon H, Elton SM, Parinandi NL. (2013). Regulation of the MIR155 host gene in physiological and pathological processes. Gene. 2013 Dec 10;532(1):1-12.
  43. Kodali ST, Kauffman P, Kotha SR, Yenigalla A, Veeraraghavan R, Pannu SR, Hund TJ, Satoskar AR, McDaniel JC, Maddipati RK, Parinandi NL. Oxidative Lipidomics: Analysis of Oxidized Lipids and Lipid Peroxidation in Biological Systems with Relevance to Health and Disease. 2020 Aug 9. In: Berliner LJ, Parinandi NL, editors. Measuring Oxidants and Oxidative Stress in Biological Systems: Springer; 2020. Chapter 5. PMID: 33411442.
  44. Oliver PJ, Arutla S, Yenigalla A, Hund TJ, Parinandi NL. Lipid Nutrition in Asthma. Cell Biochem Biophys. 2021 Sep;79(3):669-694.
  45. Fu P, Ramchandran R, Sudhadevi T, Kumar PPK, Krishnan Y, Liu Y, Zhao Y, Parinandi NL, Harijith A, Sadoshima J, Natarajan V. NOX4 Mediates Pseudomonas aeruginosa-Induced Nuclear Reactive Oxygen Species Generation and Chromatin Remodeling in Lung Epithelium. Antioxidants (Basel). 2021 Mar 17;10(3):477.
  46. Nirujogi TS, Kotha SR, Chung S, Reader BF, Yenigalla A, Zhang L, Shapiro JP, Wisler J, Christman JW, Maddipati K, Parinandi NL, Karpurapu M. Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. J Innate Immun. 2022;14(5):555-568.
  47. Parinandi N, Gerasimovskaya E, Verin A. Editorial: Molecular mechanisms of lung endothelial permeability. Front Physiol. 2022 Jul 22;13:976873. doi: 10.3389/fphys.2022.976873.

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