This Core expands the capabilities of neuroscience research on campus by making available specialized expertise, equipment and assistance to support electrophysiological analyses of signal transduction and synaptic function in cultured cells, tissue slices and zebrafish embryos.
Core Director
Candice Askwith, PhD
Candice.Askwith@osumc.edu
Core Manager
Fangli Zhao
Fangli.Zhao@osumc.edu
614-688-7943
Location
Graves Hall
Available services
- Technical expertise, equipment and consultation for conducting electrophysiological studies
- Extracellular or patch clamp recordings to screen for alterations in synaptic transmission, such as measurement of EPSP or EPSC or alteration in LTP or LTD, in cerebellar, hippocampal, hypothalamic and spinal cord slices
- Electrophysiological studies of voltage- and ligand-channels in isolated or cultured neurons
User fees
Service
- LTP or LTD (2 animals /day): $350 /day
- Whole cell patch clamping: $375 /day
- Specialized recording: $350 /day
- Training: $50 /day
- Data analysis: $50 /day
- Consultation: No charge
Equipment rental
- Extracellular rig: $50 /day or use
- Whole cell slice rig: $100 /day or use
- Whole cell rig: $25 /day or use
Electrophysiology core policies
Purpose
The primary objective of the Electrophysiology Core is to provide critical support for neuroscience projects that require analysis of ion channel activity and electrophysiology in neurons and glia. The Core will perform such experiments for pilot and existing projects and will also provide training and access to equipment for users to perform their own experiments.
Initiation of projects
All projects are to be initiated at the discretion of the Core Director. A decision to accept projects is dependent on the expertise, equipment, capacity, and workload of the Core. If scheduling conflicts arise, priority will be given to projects in the Department of Neuroscience and to PIs that have included a portion of the core staff on their funded projects. Individuals interested in using the Core contact the Core Director to set up an initial consultation.
Services
The primary service of the Electrophysiology Core is to provide expert consultation and assistance with electrophysiological analyses of ion channel activity in neuronal cells and tissues. Typically, this involves assessment of neuronal excitability and action potential firing, measurements of synaptic transmission, or recordings of the activity of specific ligand-gated or voltage-gated ion channels. The core can perform experiments for the User and can also provide access to equipment in the form of stations (or rigs), training in the use of this equipment, and consultation on experimental design and data analysis.
User responsibilities
The User is responsible for the cost of all unique reagents required for the project. The Core will provide access to common consumables, inhibitors, and chemicals. The User is also responsible for identifying an individual to implement the project and/or be trained by Core Staff. In the event that Core staff perform the experiments directly, the User and Core Director will establish a minimal and maximal cost for the project up front, as well as make arrangements (where applicable), regarding (co-) authorships and/or intellectual property issues. The User agrees to acknowledge the Neuroscience Electrophysiology Core in all publications related to data acquired with core equipment, data that were dependent on training or consultation by Core staff, or data provided to the User by the Core. Scheduling of experiments will be done through consultation between the Core Director, the User, and Core Manager. Once scheduled, the appropriate time and equipment will be reserved for the User and changes in scheduling within 3 days of projected use will result in billing for full services.
The User acknowledges that the Core reserves the right to halt or cancel experiments if:
- a minimal likelihood of completion is indicated by the results obtained,
- core equipment is consistently used inappropriately causing or increasing the likelihood of damage,
- the User fails to maintain a safe and clean work area within the Core,
- the User fails to provide requested information to the Core Director or Core personnel regarding experimental reagents, animals, animal protocols, BSL status, or recombinant DNA permission,
- the feasibility of attaining the experimental goals become unrealistic, or
- non-adherence by the User to proper personal conduct and/or violation of university rules/procedures. Proper conduct requires that a comfortable work environment be maintained within the core and personnel are treated with respect at all times: abusive language, harassment, or threats to personnel will be considered non-adherence to proper personal conduct.
Selected publications
Miller LN, Walters AE, Denninger JK, Hanson MA, Marshall AH, Johantges AC, Hosawi M, Sebring G, Rieskamp JD, Ding T, Rindani R, Chen KS, Goldberg ME, Senthilvelan S, Volk A, Zhao F, Askwith C, Wester JC, Kirby ED.
Cystic fibrosis macrophage function and clinical outcomes after elexacaftor/tezacaftor/ivacaftor.
Zhang S, Shrestha CL, Robledo-Avila F, Jaganathan D, Wisniewski BL, Brown N, Pham H, Carey K, Amer AO, Hall-Stoodley L, McCoy KS, Bai S, Partida-Sanchez S, Kopp BT. Eur Respir J. 2023 Apr 1;61(4):2102861. doi: 10.1183/13993003.02861-2021. PMID: 36265882; PMCID: PMC10066828.
Short-term high-fat diet consumption impairs synaptic plasticity in the aged hippocampus via IL-1 signaling.
González Olmo BM, Bettes MN, DeMarsh JW, Zhao F, Askwith C, Barrientos RM. NPJ Sci Food. 2023 Jul 17;7(1):35. doi: 10.1038/s41538-023-00211-4. PMID: 37460765
Neural stem and progenitor cells support and protect adult hippocampal function via vascular endothelial growth factor secretion.
Miller LN, Walters AE, Denninger JK, Hanson MA, Marshall AH, Johantges AC, Hosawi M, Sebring G, Rieskamp JD, Ding T, Rindani R, Chen KS, Goldberg ME, Senthilvelan S, Volk A, Zhao F, Askwith C, Wester JC, Kirby ED. Mol Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8. Online ahead of print. PMID: 39528687
Tapp ZM, Cornelius S, Oberster A, Kumar JE, Atluri R, Witcher KG, Oliver B, Bray C, Velasquez J, Zhao F, Peng J, Sheridan J, Askwith C, Godbout JP, Kokiko-Cochran ON. Exp Neurol. 2022 Jul;353:114058. doi: 10.1016/j.expneurol.2022.114058. Epub 2022 Mar 28. PMID: 35358498; PMCID: PMC9068267.
Disruption of dopamine receptor 1 localization to primary cilia impairs signaling in striatal neurons.
Stubbs T, Koemeter-Cox A, Bingman JI, Zhao F, Kalyanasundaram A, Rowland LA, Periasamy M, Carter CS, Sheffield VC, Askwith CC, Mykytyn K. J Neurosci. 2022 Jul 25;42(35):6692–705. doi: 10.1523/JNEUROSCI.0497-22.2022. Epub ahead of print. PMID: 35882560; PMCID: PMC9436016.
Chronic Cortical Inflammation, Cognitive Impairment, and Immune Reactivity Associated with Diffuse Brain Injury Are Ameliorated by Forced Turnover of Microglia.
Bray CE, Witcher KG, Adekunle-Adegbite D, Ouvina M, Witzel M, Hans E, Tapp ZM, Packer J, Goodman E, Zhao F, Chunchai T, O'Neil S, Chattipakorn SC, Sheridan J, Kokiko-Cochran ON, Askwith C, Godbout JP. J Neurosci. 2022 May 18;42(20):4215-4228. doi: 10.1523/JNEUROSCI.1910-21.2022. Epub 2022 Apr 19. PMID: 35440489; PMCID: PMC9121837.
In vivo Mouse Intervertebral Disc Degeneration Models and Their Utility as Translational Models of Clinical Discogenic Back Pain: A Comparative Review.
Tang SN, Walter BA, Heimann MK, Gantt CC, Khan SN, Kokiko-Cochran ON, Askwith CC, Purmessur D. Front Pain Res (Lausanne). 2022 Jun 22;3:894651. doi: 10.3389/fpain.2022.894651. PMID: 35812017; PMCID: PMC9261914.
CFTR Modulators Restore Acidification of Autophago-Lysosomes and Bacterial Clearance in Cystic Fibrosis Macrophages.
Badr A, Eltobgy M, Krause K, Hamilton K, Estfanous S, Daily KP, Abu Khweek A, Hegazi A, Anne MNK, Carafice C, Robledo-Avila F, Saqr Y, Zhang X, Bonfield TL, Gavrilin MA, Partida-Sanchez S, Seveau S, Cormet-Boyaka E, Amer AO. Front Cell Infect Microbiol. 2022 Feb 16;12:819554. doi: 10.3389/fcimb.2022.819554. PMID: 35252032; PMCID: PMC8890004.
TBI causes chronic cortical inflammation and neuronal dysfunction mediated by microglia.
Witcher K, Bray C, Chunchai T, Zhao F, O’Neil S, Gordillo A, Campbell A, McKim D, Liu X, Dziabis J, Quan N, Eiferman, D, Fischer A, Kokiko-Cochran O, Askwith C, Godbout J
J Neurosci. 2021 Jan 12;JN-RM-2469-20. doi: 10.1523/JNEUROSCI.2469-20.2020.
Translational regulation in the brain by TDP-43 phase separation.
Gao J, Wang L, Ren X, Dunn JR, Peters A, Miyagi M, Fujioka H, Zhao F, Askwith C, Liang J, Wang X. J Cell Biol. 2021 Oct 4;220(10):e202101019. doi: 10.1083/jcb.202101019. Epub 2021 Aug 24. PMID: 34427634
Wang X, Xu Z, Zhao F, Lin KJ, Foster JB, Xiao T, Kung N, Askwith CC, Bruno JP, Valentini V, Hodgetts KJ, Lin CG.
Neurobiol Stress. 2020 Jul 13;13:100240. doi: 10.1016/j.ynstr.2020.100240. eCollection 2020 Nov.
PMID: 33344696 Free PMC article.
Insulin Modulates Excitatory Synaptic Transmission and Synaptic Plasticity in the Mouse Hippocampus.
Zhao F, Siu JJ, Huang W, Askwith C, Cao L.
Neuroscience. 2019 Jul 15;411:237-254. doi: 10.1016/j.neuroscience.2019.05.033. Epub 2019 May 28
Enhancement of tripartite synapses as a potential therapeutic strategy for Alzheimer's disease: a preclinical study in rTg4510 mice.
Foster JB, Lashley R, Zhao F, Wang X, Kung N, Askwith CC, Lin L, Shultis MW, Hodgetts KJ, Lin CG.
Alzheimers Res Ther. 2019 Aug 23;11(1):75. doi: 10.1186/s13195-019-0530-z.
PMID: 31439023. PMCID: PMC6706914
Dissipation of transmembrane potassium gradient is the main cause of cerebral ischemia-induced depolarization in astrocytes and neurons.
Du Y, Wang W, Lutton AD, Kiyoshi CM, Ma B, Taylor AT, Olesik JW, McTigue DM, Askwith CC, Zhou M.
Exp Neurol. 2018 May;303:1-11. doi: 10.1016/j.expneurol.2018.01.019. Epub 2018 Feb 3. PMID: 29407729
Pyridazine-derivatives Enhance Structural and Functional Plasticity of Tripartite Synapse Via Activation of Local Translation in Astrocytic Processes.
Foster JB, Zhao F, Wang X, Xu Z, Lin K, Askwith CC, Hodgetts KJ, Lin CG.
Neuroscience. 2018 Sep 15;388:224-238. doi: 10.1016/j.neuroscience.2018.07.028. Epub 2018 Jul 27.
PMID: 30056115
Syncytial isopotentiality: A system-wide electrical feature of astrocytic networks in the brain.
Kiyoshi CM, Du Y, Zhong S, Wang W, Taylor AT, Xiong B, Ma B, Terman D, Zhou M.
Glia. 2018 Dec;66(12):2756-2769. doi: 10.1002/glia.23525. Epub 2018 Sep 12.
PMID: 30277621.