The Main Campus Imaging Core provides training, expertise and equipment to perform fluorescence confocal microscopy. This facility provides a well-maintained point-scanning confocal microscope and provides training to users to establish independent imaging experiments.

Core Director
Andy Fischer, PhD
Andrew.Fischer@osumc.edu

Core Manager
Lisa Kelly
Lisa.Kelly@osumc.edu

Location
Graves Hall

Leica Confocal

The Leica SP8 is an upright point-scanning confocal suitable for routine confocal imaging of fixed specimens.

Leica Confocal

Features:

  • Upright fully motorized with 10X, 20X, 40X and 63X high NA objectives
  • 40x and 63x are oil immersion lenses
  • Motorized XYZ stage with XY stitching
  • 4 excitation lines (405, 488, 561 and 640 nm) from solid state lasers and AOTFs
  • Adjustable filters for emission spectra
  • 2 emission PMTs, 1 HyD emission detector, and 1 transmitted light PMT
  • Digital deconvolution
  • LASX suite for Z-stack projections, rotations, orthogonal projections and rotation movies  

Olympus Multi-photon

The Olympus FVMPE-RS is an upright multi-photon microscope capable of confocal fluorescence imaging deep into tissue in brain slices, anesthetized or unanesthetized mice.

Features:

  • Dual scan head
  • MPE Objectives: 60X dipping, 25X immersion, 20X air and 5X air (MPE Objectives are designed for deep imaging-up to 8 mm working distance/depth)
  • Detectors: PMT and GAASP
  • High-Speed Scanning at up to 438 Frames-per-Second
  • Independent Photostimulation Control
  • Microsecond Timing for Electrophysiology and Optogenetics
  • Automated Laser Alignment
  • FluoView Software
  • Coherent Twin Chameleon laser system: 700-1060 nm
  • Prior XY motorized stage 

Neurotar Home Cage Arena (for in vivo imaging of unanesthetized mice). 

Multiphoton machine cover off Multiphoton machine cover on

 

 

 

 

 

 

 

 

Two-Photon Microscope Director
Karl Obrietan, PhD
Obrietan.1@osu.edu

User Fees

To be determined