The Main Campus Imaging Core provides training, expertise and equipment to perform fluorescence confocal microscopy. This facility provides well-maintained point-scanning confocal and multiphoton microscopes and provides training to users to establish independent imaging experiments.
Core Director
Andy Fischer, PhD
Andrew.Fischer@osumc.edu
Core Manager
Lisa Kelly
Lisa.Kelly@osumc.edu
Location
Graves Hall
Leica confocal
The Leica SP8 is an upright point-scanning confocal suitable for routine confocal imaging of fixed specimens.
Features:
- Upright fully motorized with 10X, 20X, 40X and 63X high NA objectives
- 40x and 63x are oil immersion lenses
- Motorized XYZ stage with XY stitching
- 4 excitation lines (405, 488, 561 and 640 nm) from solid state lasers and AOTFs
- Adjustable filters for emission spectra
- 2 emission PMTs, 1 HyD emission detector, and 1 transmitted light PMT
- Digital deconvolution
- LASX suite for Z-stack projections, rotations, orthogonal projections and rotation movies
Following in person training on the system users will be granted access to the online calendar to reserve the system and card swipe access to the shared equipment room in Graves Hall. Please note that this is a heavily used shared resource. Thus, during normal working hours reservations are restricted to 4hr blocks of time per lab.
Olympus multi-photon
The Olympus FVMPE-RS is an upright multi-photon microscope capable of confocal fluorescence imaging deep into tissue in brain slices, anesthetized or unanesthetized mice.
Features:
- Dual scan head
- MPE Objectives: 60X dipping, 25X immersion, 20X air and 5X air (MPE Objectives are designed for deep imaging-up to 8 mm working distance/depth)
- Detectors: PMT and GAASP
- High-Speed Scanning at up to 438 Frames-per-Second
- Independent Photostimulation Control
- Microsecond Timing for Electrophysiology and Optogenetics
- Automated Laser Alignment
- FluoView Software
- Coherent Twin Chameleon laser system: 700-1060 nm
- Prior XY motorized stage
- Neurotar Home Cage Arena (for in vivo imaging of unanesthetized mice).
User fees
Cost effective as of July 1, 2024.
Confocal user
- Department of Neuroscience labs: $25/ hour
- Other labs: $35/ hour