The Main Campus Imaging Core provides training, expertise and equipment to perform fluorescence confocal microscopy. This facility provides a well-maintained point-scanning confocal microscope and provides training to users to establish independent imaging experiments.
Andy Fischer, PhD
The Leica SP8 is an upright point-scanning confocal suitable for routine confocal imaging of fixed specimens.
- Upright fully motorized with 10X, 20X, 40X and 63X high NA objectives
- 40x and 63x are oil immersion lenses
- Motorized XYZ stage with XY stitching
- 4 excitation lines (405, 488, 561 and 640 nm) from solid state lasers and AOTFs
- Adjustable filters for emission spectra
- 2 emission PMTs, 1 HyD emission detector, and 1 transmitted light PMT
- Digital deconvolution
- LASX suite for Z-stack projections, rotations, orthogonal projections and rotation movies
The Olympus FVMPE-RS is an upright multi-photon microscope capable of confocal fluorescence imaging deep into tissue in brain slices, anesthetized or unanesthetized mice.
- Dual scan head
- MPE Objectives: 60X dipping, 25X immersion, 20X air and 5X air (MPE Objectives are designed for deep imaging-up to 8 mm working distance/depth)
- Detectors: PMT and GAASP
- High-Speed Scanning at up to 438 Frames-per-Second
- Independent Photostimulation Control
- Microsecond Timing for Electrophysiology and Optogenetics
- Automated Laser Alignment
- FluoView Software
- Coherent Twin Chameleon laser system: 700-1060 nm
- Prior XY motorized stage
Neurotar Home Cage Arena (for in vivo imaging of unanesthetized mice).
Two-Photon Microscope Director
Karl Obrietan, PhD
To be determined