Single Cell Omics Core

The Single Cell Omics Core facilitates the generation and analyses of single cell libraries to produce publication quality data. Expertise with scRNA-seq, scATAC-seq and multi-omics.

Core Director
Andy Fischer, PhD
Andrew.Fischer@osumc.edu

Core Manager
Heithem El-Hodiri
Heithem.El-Hodiri@osumc.edu
614-292-1226

Location
Graves Hall 3187

Available services and equipment

Services

• Consulting – experiment design strategies, best reagents, techniques, sequencing strategies
• 10X genomics or Curio Bioscience Spatial Transcriptomics
• Cell dissociation and/or nuclei preparations
• Library preparation – loading cells into Cell Controller, tagmentation, clean-up, prep for sequencing
• Bioinformatics – help with OSC supercomputer for 10X CellRanger
• Bioinformatic analyses – Seurat, Monocle, Signac, CellChat, SingleCellSignalR, motif analyses, SoupOrCell, different Integration strategies, overlays of query libraries onto establishedtrajectories, GO enrichment analyses, etc.

Equipment

• 10X Chromium Cell Controller
• 10X Chromium X Controller
• BioRad PCR machine
• Miltenyi OCTO tissue dissociator
• Miltenyi MACs
• Countess II automated cell counter
• 4 Dell/Apple Workstations
• Heating blocks, mini-spin, refrigerated centrifuge, approved pipettemen

User fees

Costs effective as of July 1, 2024.

All users

• Flat rate (trained users): $100/library
• Flat rate (new users, includes training): $250/library
• Library preparation: $400/library