Single Cell Omics Core

The Single Cell Omics Core facilitates the generation and analyses of single cell libraries to produce publication quality data. Expertise with scRNA-seq, scATAC-seq and multi-omics.

Core Director
Andy Fischer, PhD
Andrew.Fischer@osumc.edu

Core Manager
Heithem El-Hodiri
Heithem.El-Hodiri@osumc.edu
614-292-1226

Location
Graves Hall 3187

Available services and equipment

Services

  • Consulting – experiment design strategies, best reagents, techniques, sequencing strategies
  • 10X genomics or Curio Bioscience Spatial Transcriptomics
  • Cell dissociation and/or nuclei preparations
  • Library preparation – loading cells into Cell Controller, tagmentation, clean-up, prep for sequencing
  • Bioinformatics – help with OSC supercomputer for 10X CellRanger
  • Bioinformatic analyses – Seurat, Monocle, Signac, CellChat, SingleCellSignalR, motif analyses, SoupOrCell, different Integration strategies, overlays of query libraries onto establishedtrajectories, GO enrichment analyses, etc.

Equipment

  • 10X Chromium Cell Controller
  • 10X Chromium X Controller
  • BioRad PCR machine
  • Miltenyi OCTO tissue dissociator
  • Miltenyi MACs
  • Countess II automated cell counter
  • 4 Dell/Apple Workstations
  • Heating blocks, mini-spin, refrigerated centrifuge, approved pipettemen

Single cell lab equipment Single cell lab equipment machine


User fees

Costs effective as of July 1, 2024.

All users

  • Flat rate (trained users): $100/library
  • Flat rate (new users, includes training): $250/library
  • Library preparation: $400/library

Single Cell Omics core policies

Selected publications

Examples of publications that have benefitted from single cell omics core services.