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Rita Alevriadou, PhD

B. Rita Alevriadou, PhD
Associate Professor of Biomedical Engineering and Internal Medicine
Cardiovascular Medicine, Department of Internal Medicine, The Ohio State University
Phone: (614) 292-5160
 
 
 
 
 
 
The goal of the Alevriadou laboratory (Vascular Bioengineering Laboratory, DHLRI 665) is to delineate the role of oxidative stress and mechanical forces (shear stress) in endothelial cell (EC) intracellular signal transduction, gene expression and protein synthesis in an effort to better understand, prevent and treat cardiovascular disease (ischemia/reperfusion injury, atherosclerosis, inflammation, thrombosis). Previous work of ours showed that cultured EC exposure to mechanical shear stress reproduces certain aspects of the reperfusion-induced endothelial injury, namely the nitric oxide (NO)/peroxynitrite-mediated inhibition of respiration and mitochondrial production of reactive oxygen species (mtROS). More recently, we discovered that the shear-induced NO, the cellular and, in particular, mtROS and the mitochondrial fission protein dynamin-related protein 1 (Drp1) are all responsible for fragmentation of the mitochondrial network in cultured ECs exposed to simulated ischemia/reperfusion (I/RP), which may be the underlying cause of the EC dysfunction in postischemic hearts. Our current work concentrates on the EC mitochondrial morphological changes (mitochondrial fusion/fission and motility) and the mitochondrial autophagic processes (mitophagy) following hypoxia/reoxygenation or simulated I/RP, in an effort to better understand the molecu¬lar mechanisms that regulate the endothelial dysfunction (death vs. survival) in postischemic hearts and the ischemia-induced angiogenesis.
Team Members
Devi M. Nair, PhD, Postdoctoral Researcher
Randy J. Giedt, MS, PhD candidate
Christopher Scheitlin, BS, PhD candidate
Shuting Zhao, MS, Lab Volunteer
Colton Lloyd, BS candidate, Research Assistant
Research Interests
Vascular biology/bioengineering
Endothelial cell mechanosignaling (shear stress) & function (survival, angiogenesis)
Free radicals/oxidative stress
Mitochondrial biology (dynamics, motility, autophagy/mitophagy)
Hypoxia/reoxygenation & ischemia/reperfusion
Molecular mechanisms of blood cell adhesion (inflammation, thrombosis)
Selected Publications
Giedt RJ, Yang C, Zweier JL, Matzavinos A, Alevriadou BR. Mitochondrial fission in endothelial cells after simulated ischemia/reperfusion: Role of nitric oxide and reactive oxygen species. Free Radic Biol Med. 2012 Jan;52(2):348-56.

Chen J, Chen CL, Alevriadou BR, Zweier JL, Chen YR. Excess NO predisposes mitochondrial succinate-cytochrome c reductase to produce hydroxyl radical. Biochim Biophys Acta. 2011 May;1807(5):491-502.

Alevriadou BR, Jones CI 3rd, Giedt RJ. Nitric oxide and endo¬thelial mitochondrial function: Implications for ischemi¬a/reper¬fusion. In: Hsiai TK, Blackman B, Jo H, eds. Hemody¬namics & Mecha¬nobiolo¬gy of Endotheli¬um. Singapore & USA: World Scien¬tific Publishing Co., 2010 Sep;pp.153-77.

Lyczkowski RW, Alevriadou BR, Horner M, Panchal CB, Shroff SG. Application of multiphase computational fluid dynamics to analyze monocyte adhesion. Ann Biomed Eng. 2009 Aug;37(8):1516-33.

Han Z, Varadharaj S, Giedt RJ, Zweier JL, Szeto HH, Alevriadou BR. Mitochondria-derived reactive oxygen species mediate heme oxygenase-1 expression in sheared endothelial cells. J Pharmacol Exp Ther. 2009 Apr;329(1):94-101.

Jones CI 3rd, Han Z, Presley T, Varadharaj S, Zweier JL, Ilangovan G, Alevriadou BR. Endothelial cell respiration is affected by the oxygen tension during shear exposure: role of mitochondrial peroxynitrite. Am J Physiol Cell Physiol. 2008 Jul;295(1):C180-91.

Jones CI 3rd, Zhu H, Martin SF, Han Z, Li Y, Alevriadou BR. Regulation of antioxidants and phase 2 enzymes by shear-induced reactive oxygen species in endothelial cells. Ann Biomed Eng. 2007 May;35(5):683-693.

Han Z, Chen YR, Jones CI 3rd, Meenakshisundaram G, Zweier JL, Alevriadou BR. Shear-induced reactive nitrogen species inhibit mitochondrial respiratory complex activities in cultured vascular endothelial cells. Am J Physiol Cell Physiol. 2007 Mar;292:C1103-C1112.
 
Martin SF, Chatterjee S, Parinandi N, Alevriadou BR. Rac1 inhibition protects against hypoxia/reoxygenation-induced lipid peroxidation in human vascular endothelial cells. Vascul Pharmacol. 2005 Sept;43(3):148-156.

Haun JB, Baldwin WM 3rd, Alevriadou BR. Clearance of complement by human vascular endothelial cells: Effects of hypoxia/reoxygenation and IL-1β activation. Transpl Int. 2005 Apr;18:475-482.